Human Anti-Carcinoembryonic Antigen / CD66 Antibody Product Attributes
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 min at RT)
Anti-Carcinoembryonic Antigen / CD66 Antibody Clone: SPM551
Clone SPM551 Host and Isotype: Mouse IgG1 kappa
Anti-Human Carcinoembryonic Antigen / CD66 Positive Control Sample: MCF7 or 293T cells. Colon carcinoma
Cellular Localization of Antibody
Cytoplasmic, luminal surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human recombinant CEA protein
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
Carcinoembryonic Antigen / CD66 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:Anti-CEACAM5 antibody staining is expected to be primarily localized to the plasma membrane.
Observed Antibody Staining Data By Tissue Type:Variations in Carcinoembryonic Antigen / CD66 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the appendix, colon, rectum. More moderate antibody staining intensity was present in glandular cells in the appendix, colon, rectum. Low, but measureable presence of Carcinoembryonic Antigen / CD66 could be seen inepidermal cells in the skin, glandular cells in the gallbladder and salivary gland, hematopoietic cells in the bone marrow, hepatocytes in liver, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the cervix and uterine, tonsil and vagina. We were unable to detect Carcinoembryonic Antigen / CD66 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of Carcinoembryonic Antigen / CD66 expression as measured by anti-Carcinoembryonic Antigen / CD66 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Limitations and Warranty
enQuire Bio's Carcinoembryonic Antigen / CD66 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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