Immunohistochemical staining of Carcinoembryonic  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Carcinoembryonic [COL-1] Antibody (cGMP).

$ 158.00$ 388.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD52
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG2a, kappa

Product NumberDescriptionPrice
QIVD52-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD52-0.5ml Size: 0.5 ml, Format: Concentrate $ 205.00
QIVD52-1ml Size: 1 ml, Format: Concentrate $ 345.00
QIVD52-6ml Size: 6 ml, Format: Predilute $ 235.00
Shipping Information
In Stock
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QIVD52-0.1ml
Carcinoembryonic General Information
Alternate Names
Molecular Weight
76.8 kDa
Chromosomal Location
q13.2 [chr: 19] [chr_start: 41708585] [chr_end: 41729798] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolCEACAM5
Entrez Gene ID1048
RefSeq Protein Accession(s)NP_001278413; NP_001295327; NP_004354
RefSeq mRNA Accession(s)XM_017026145; XM_017026146; XM_011526322; NM_001291484; NM_001308398; NM_004363
RefSeq Genomic Accession(s)NC_000019
UniProt ID(s)P06731
PharmGKB ID(s)PA26361
KEGG Gene ID(s)hsa:1048
General Description of Carcinoembryonic .
This antibody is a member of a family of complex antigens, which also includes non-specific cross-reacting antigens (NCA). This antibody stains specific regions of CEA. It reacts with colorectal adenocarcinomas but does not show any staining with polymorphonuclear neutrophils and erythrocytes.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:75-1:100
Pretreatment No Pretreatment Required
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Carcinoembryonic Antibody Clone: COL-1
Host and Isotype: Mouse IgG2a, kappa
Recommended Positive Control Sample: Colon Carcinoma
Cellular Localization of Antibody COL-1 Staining: Cytoplasmic and lumenal membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with extract of colon carcinoma cells.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Carcinoembryonic Information for Pathologists

Summary:

Also called CEA, carcinoembryonic antigen and CEACAM5. Either polyclonal (pCEA) or monoclonal (mCEA). Normally detected in glycocalyx of fetal epithelial cells. May play a role in the metastasis of cancer cells. Usually considered an epithelial marker with strong staining in adenocarcinomas.

Common Uses By Pathologists:

Adenocarcinoma (lung) vs. epithelioid mesothelioma: monoclonal CEA is 97% specific for lung adenocarcinoma (Histopathology 2006;48:223); exhibits diffuse cytoplasmic staining with membrane enhancement in adenocarcinoma, negative in mesothelioma. Adenocarcinoma (lung) vs. reactive mesothelial cells in fluid cytology: positive staining is 76% sensitive (Am J Clin Pathol 2001;116:709). Breast cancer vs. benign breast disease in FNA fluid: high levels are suggestive of malignancy (Arch Pathol Lab Med 2004;128:1251, free full text). Colorectal carcinoma: monitor serum levels (elevated in 72-97%) to detect recurrence (World J Gastroenterol 2006;12:3891), elevated preoperative serum levels are poor prognostic factor (Int J Cancer 2002;101:545); caution - elevated levels also present in cirrhosis, biliary obstruction, hepatitis, inflammatory bowel disease, smokers and post-surgical bowel sequestration with mucocele (Arch Pathol Lab Med 2003;127:1376). Cysts (various): CEA levels over 5 ng/dl in ascites fluid are associated with malignancy (J Clin Pathol 2001;54:831); but fluid in cysts may be CEA+ even when benign (Mod Pathol 1998;11:1171).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

There are no reviews yet.

Be the first to review “Carcinoembryonic [COL-1] Antibody (cGMP).”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Chat: See lower right corner. | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Carcinoembryonic [COL-1] Antibody (cGMP).