Analysis of Mass Spec data (dashed-line) of fractions stained with Catenin, beta MS-QAVA™ monoclonal antibody [Clone: 6F9] (solid-line), reveals that less than 5.2% of signal is attributable to non-specific binding of anti-Catenin, beta [Clone: 6F9] to targets other than CTNNB1 protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in A431, RT4 and MCF7 cells.
Analysis of Mass Spec data (dashed-line) of fractions stained with Catenin, beta MS-QAVA™ monoclonal antibody [Clone: 6F9] (solid-line), reveals that less than 5.2% of signal is attributable to non-specific binding of anti-Catenin, beta [Clone: 6F9] to targets other than CTNNB1 protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in A431, RT4 and MCF7 cells.

Anti-Catenin, beta Antibody Clone 6F9

$ 118.00$ 418.00
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SKU: 1499-MSM5
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: MS-QAVA™ Validated

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Datasheets and Documentation
Product Datasheet
SKU: 1499-MSM5-P0
Catenin, beta General Information
Alternate Names
Cadherin associated protein, Catenin beta-1, ?-catenin, beta catenin CTNNB1, CTNNB1
Molecular Weight
92kDa
Chromosomal Location
3p22.1
Curated Database and Bioinformatic Data
Gene SymbolCTNNB1
Entrez Gene ID1499
Ensemble Gene IDENSG00000168036
RefSeq Protein Accession(s)XP_016861227, NP_001091679, XP_005264943, NP_001091680, XP_006713046, XP_006713047, NP_001317658, XP_006713048, NP_001895
RefSeq mRNA Accession(s)XM_006712984, XM_006712983, NM_001904, XM_017005738, NM_001098209, NM_001330729, NM_001098210, XM_006712985
RefSeq Genomic Accession(s)NC_018914, NG_013302, NC_000003
UniProt ID(s)A0A024R2Q3, P35222, B4DGU4
UniGene ID(s)A0A024R2Q3, P35222, B4DGU4
HGNC ID(s)2514
Cosmic ID(s)CTNNB1
KEGG Gene ID(s)hsa:1499
PharmGKB ID(s)PA27013
General Description of Catenin, beta.
This MAb recognizes a protein of 92kDa, which is identified as beta-catenin. It shows no cross-reaction with gamma-catenin (also known as plakoglobin). The catenins, alpha, beta and gamma bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. Alpha-catenin was initially described as an E-cadherin-associated protein, and has been shown to associate with other members of the cadherin family, such as N-cadherin and P-cadherin. Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. Beta-catenin has also been found in complexes with the tumor suppressor protein APC.

Human Anti-Catenin, beta Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC). Additionally, this antibody has been validated by MS-QAVA™. Specificity has been quantitatively determined via Mass Spec.
Clonality: Monoclonal
Anti-Catenin, beta Antibody Clone: 6F9
Clone 6F9 Host and Isotype: Mouse IgG1 kappa
Anti-Human Catenin, beta Positive Control Sample: HeLa or MCF-7 cells. Breast carcinoma
Cellular Localization of Antibody Cell surface and cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant chicken ?-catenin
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Catenin, beta Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Variations in Beta Catenin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in bile duct cells in the liver, cells in the seminiferous ducts in testis, cells in the tubules in kidney, epidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the adrenal gland, appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, hepatocytes in liver, keratinocytes in skin, myocytes in heart muscle, myoepithelial cells in the breast, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in bile duct cells in the liver, cells in the seminiferous ducts in testis, cells in the tubules in kidney, epidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the adrenal gland, appendix, breast, cervix, uterine, colon, duodenum, endometrium, epididymis, fallopian tube, gallbladder, parathyroid gland, prostate, rectum, salivary gland, seminal vesicle, small intestine, stomach and thyroid gland, hepatocytes in liver, keratinocytes in skin, myocytes in heart muscle, myoepithelial cells in the breast, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina, trophoblastic cells in the placenta and urothelial cells in the urinary bladder. Low, but measureable presence of Beta Catenin could be seen in cells in the endometrial stroma in endometrium, cells in the glomeruli in kidney, cells in the molecular layer in cerebellum, endothelial cells in the cerebral cortex, fibroblasts in skin, glial cells in the caudate nucleus, cerebral cortex and hippocampus, hematopoietic cells in the bone marrow, Leydig cells in the testis, neuronal cells in the caudate nucleus, cerebral cortex and hippocampus, ovarian stroma cells in the ovary and Purkinje cells in the cerebellum. We were unable to detect Beta Catenin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Type:

Tissues from cancer patients, for instance, have their own distinct pattern of Beta Catenin expression as measured by anti-Beta Catenin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ ++ +++ +++ +++ + +++ +++ +++ - +++ +++ +++ +++ ++ +++ ++ + +++ +++
CTNNB1 Variability ++ ++ ++ + + +++ ++ ++ ++ + + ++ + + ++ + ++ ++ + ++

Limitations and Warranty

This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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