Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)||1:100-1:200|
|Pretreatment||Citrate Buffer pH 6.0|
|Incubation Parameters||30 min at Room Temperature|
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-Catenin, beta Antibody Clone: Polyclonal
Host and Isotype: Rabbit Undetermined
Recommended Positive Control Sample: Breast carcinoma, Large intestine
Cellular Localization of Antibody Polyclonal Staining: Cytoplasmic, cell membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide derived from the C-terminus of human beta-catenin protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Catenin, beta Information for Pathologists
Summary:Protein encoded by CTNNB1 gene, coordinates cell-cell adhesion and gene transcription (Wikipedia: Beta Catenin [Accessed 18 December 2018]). Pathophysiology Part of Wnt signalling pathway, highly conserved pathway with critical role in embryologic development (Dev Cell 2009;17:9), carcinogenesis and epithelial-to-mesenchymal transition. Upon Wnt activation, ? catenin is translocated from membrane (where it interacts with E-cadherin) to cytoplasm and nucleus, where it interacts with transcriptional activators. Clinical features
Notable Clinical Features:Mutations and overexpression of ? catenin are associated with various carcinomas. Colon: plays a critical role in tumorigenesis (mutations in APC or ? catenin present in 90% of colon cancers). Thyroid: pathway important for anaplastic and possibly papillary thyroid carciomas (Front Endocrinol (Lausanne) 2012;3:31). Uterus: endometrioid endometrial carcinoma is associated with ? catenin mutations. Diagrams / tables
Common Uses By Pathologists:Interpretation: nuclear staining is significant in fibroblasts; nuclear or cytoplasmic staining is significant in epithelial cells. Classify hepatocellular adenoma subtypes and distinguish from focal nodular hyperplasia (Am J Surg Pathol 2012;36:1691, Int J Hepatol 2013;2013:268625, Hum Pathol 2013;44:750). Positive / mutated in fibromatosis (Mod Pathol 2012;25:1551). Distinguish mesenteric fibromatosis (positive with nuclear staining due to mutations in APC / ? catenin pathway causing nuclear accumulation) from GIST tumors (negative) and sclerosing mesenteritis (negative, Am J Surg Pathol 2002;26:1296). Distinguish fibromatosis (diffuse or rarely focal nuclear staining for deep tumors) from low grade fibromyxoid sarcoma and other myofibroblastic, or fibroblastic tumors / sarcomas (negative for nuclear staining, Am J Surg Pathol 2005;29:653).
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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