Human Anti-CD106 / VCAM1 Antibody Product Attributes
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Anti-CD106 / VCAM1 Antibody Clone: VCAM1/843
Clone VCAM1/843 Host and Isotype: Mouse IgG1 kappa
Anti-Human CD106 / VCAM1 Positive Control Sample: Human placenta or tonsil
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human VCAM1 protein
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
CD106 / VCAM1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-VCAM1 antibody staining is expected to be primarily localized to the cell junctions.
Observed Antibody Staining Data By Tissue Type:
Variations in CD106 / VCAM1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD106 / VCAM1 could be seen inlymphoid tissue in appendix and germinal center cells in the lymph node. We were unable to detect CD106 / VCAM1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's CD106 / VCAM1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.