Mouse Anti-CD115 Antibody [AFS98]

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SKU: QAB53
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: APC ;FITC ;PE
Isotype: Rat IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB53-APC-25ug
CD115 / CSF1R / MCSF Receptor General Information
Alternate Names
CSF1R, MCSF Receptor, CD115, C-FMS, HDLS, CSF-1R, FMS, CSFR, FIM2, M-CSF-R, CD115
Curated Database and Bioinformatic Data
Gene SymbolCsf1r
Entrez Gene ID12978
Ensemble Gene IDENSMUSG00000024621
RefSeq Protein Accession(s)XP_006525649, NP_001032948, XP_006525648, XP_006525647, XP_017173299
RefSeq mRNA Accession(s)XR_001782332, XM_006525584, XM_017317810, NM_007779, NM_001037859, XM_006525586, XM_006525585
RefSeq Genomic Accession(s)NC_000084
UniProt ID(s)Q3UKC6, P09581, Q0P635, Q6NXV8
UniGene ID(s)Q3UKC6, P09581, Q0P635, Q6NXV8
Cosmic ID(s)Csf1r
KEGG Gene ID(s)mmu:12978
General Description of CD115 / CSF1R / MCSF Receptor.
The AFS98 antibody is specific for mouse CD115, also known as Colony-Stimulating Factor-1 Receptor (CSF-1R), a 145 kDa receptor from the PDGF receptor family. Receptor activation by the ligands IL-34 or CSF-1 (M-CSF) occurs via homodimerization of CD115 and subsequent tyrosine phosphorylation and ubiquitination of intracellular domains. CD115 signaling promotes differentiation of myeloid precursors, as well as the continued regulation of proliferation, survival and function of mononuclear phagocytes, dendritic cells and osteoclasts. While IL-34 and CSF-1 may induce similar cellular responses, they are differentially expressed and as such exert complimentary actions via CD115.The AFS98 antibody may be used for identification of myeloid lineage cells by flow cytometry, and is commonly used for in vivo or in vitro neutralization of CSF-1 Receptor (use format appropriate for functional assays).

Mouse Anti-CD115 / CSF1R / MCSF Receptor Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD115 / CSF1R / MCSF Receptor Antibody Clone: AFS98
Clone AFS98 Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD115 / CSF1R / MCSF Receptor Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD115 / CSF1R / MCSF Receptor antibody reveals CD115 / CSF1R / MCSF Receptor expression is expected to be primarily localized to the plasma membrane and vesicles.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD115 / CSF1R / MCSF Receptor expression as measured by anti-CD115 / CSF1R / MCSF Receptor antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + + + + + + + + + + + ++ + - + + + + ++ +
CSF1R Variability ++ ++ ++ + + + + ++ + + + ++ ++ ++ +++ + ++ + + +

Selected References

Gautier EL, Chow A, Spanbroek R, Marcelin G, Greter M, Jakubzick C, Bogunovic M, Leboeuf M, van Rooijen N, Habenicht AJ, Merad M, and Randolph GJ. 2012. J. Immunol. 189: 2614-2624. (Flow cytometry)Nakamichi Y, Mizoguchi T, Arai A, Kobayashi Y, Sato M, Penninger JM, Yasuda H, Kato, S, DeLuca HF, Suda T, Udagawa N, and Takahashi N. Proc. Natl. Acad. Sci. 109: 10006-10011. (in vitro blocking)Okuno Y, Nakamura-Ishizu A, Kishi K, Suda T, and Kubota Y. 2011. Blood. 117: 5264-5272. (in vivo blocking)Fixley FJ, Xiong Y, Yu R Y-L, Sahai EA, Stanley ER, and Ye BH. 2005. J. Cell Sci. 118: 1873-1883. (Western blot)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD115 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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