enQuire-Bio-QAB25-APC-100Tests-anti-CD11b-antibody-10
Human peripheral blood monocytes were stained with 5 uL  (solid line) or 1 ug FITC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human peripheral blood monocytes were stained with 5 uL  (solid line) or 1 ug PE Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human peripheral blood monocytes were stained with 5 uL  .Human peripheral blood monocytes were stained with 5 uL  (solid line) or 1 ug FITC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human peripheral blood monocytes were stained with 5 uL  (solid line) or 1 ug PE Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human peripheral blood monocytes were stained with 5 uL  .

Anti-CD11b Antibody [ICRF44]

$ 79.00$ 219.00
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SKU: QAB25
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: APC ;Unconjugated ;FITC ;PE ;PE-Cy7
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB25-APC-25Tests
CD11b General Information
Alternate Names
Complement Receptor 3A, CR3A, ITGAM
Curated Database and Bioinformatic Data
Gene SymbolITGAM
Entrez Gene ID3684
Ensemble Gene IDENSG00000169896
RefSeq Protein Accession(s)XP_016878705, NP_000623, XP_011544152, NP_001139280, XP_011544153, XP_006721108
RefSeq mRNA Accession(s)XM_006721045, NM_000632, XM_011545850, XM_017023216, NM_001145808, XM_011545851, XR_950796,
RefSeq Genomic Accession(s)NC_000016, NG_011719, NC_018927
UniProt ID(s)P11215
UniGene ID(s)P11215
HGNC ID(s)6149
Cosmic ID(s)ITGAM
KEGG Gene ID(s)hsa:3684
PharmGKB ID(s)PA29949
General Description of CD11b.
The ICRF44 antibody reacts with human CD11b, also known as integrin alpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha M (CD11b) assembles with Integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1, ICAM-2, ICAM-3 and ICAM-4 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.The ICRF44 antibody is widely used as a marker for CD11b expression on macrophages, granulocytes, and subsets of NK cells. It is reported to be cross-reactive with a number of non-human species including Baboon, Chimpanzee, Cynomolgus, Rhesus and Swine.

Human Anti-CD11b Antibody Product Attributes

Species: Human
Cross Reactivity: baboon, chimpanzee, cynomolgus monkey, rhesus macaque, pig
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD11b Antibody Clone: ICRF44
Clone ICRF44 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD11b Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD11b antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow. Low, but measureable presence of CD11b could be seen in cells in the molecular layer in cerebellum, glial cells in the caudate nucleus, cerebral cortex and hippocampus and macrophages in lung. We were unable to detect CD11b in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Feng C, Zhang L, Almulki L, Faez S, Whitford M, Hafezi-Moghadam A, and Cross AS. 2011. J. Leukoc. Biol. 90:313-321. (Immunoprecipitation)Chang WLW and Barry PA. 2010. Proc. Natl. Acad. Sci. 107:22647-2652. (Flow cytometry - Rhesus macaque)Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, and Kettritz R. 2010. J. Biol. Chem. 286:7070-7081. (in vitro blocking)Moreau A, Hill M, Thebault P, Deschamps JY, Chiffoleau E, Chauveau C, Moullier P, Anegon I, Alliot-Licht B, and Cuturi MC. 2009. FASEB J. 23:3070-3077. (Flow cytometry - cynomolgus macaque)Sengoku K, Takuma N, Miyamoto T, Horikawa M, and Ishikawa M. 2004. Hum. Reprod. 19: 639-644. (Immunofluorescence microscopy)David A, Kacher Y, Specks U, and Aviram I. 2003. J. Leukoc. Biol. 74:551-557. (Western blot)Rezzonico R, Imbert V, Chicheportiche R, and Dayer J-M. 2001. Blood. 97: 2932-2940. (in vitro activation)

Limitations and Warranty

enQuire Bio's Human Anti-CD11b Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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