enQuire-Bio-QAB22-APC-100ug-anti-CD11b-antibody-10
C57Bl/6 bone marrow cells were stained with 0.125 ug APC-Cy7 Anti-Hu/Mo CD11b  .C57Bl/6 bone marrow cells were stained with 0.125 ug Biotin Anti-Hu/Mo CD11b (QAB22) (solid line) or no primary antibody (dashed line). Flow Cytometry Data from 10,000 events., followed by Streptavidin FITC.C57Bl/6 bone marrow cells were stained with 0.5 ug FITC Anti-Hu/Mo CD11b (QAB22) (solid line) or 0.5 ug FITC Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 bone marrow cells were stained with 0.25 ug PerCP-Cy5.5 Anti-Hu/Mo CD11b .C57Bl/6 bone marrow cells were stained with 0.125 ug Anti-Hu/Mo CD11b PE (QAB22) (solid line) or 0.125 ug Rat IgG2b PE isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 bone marrow cells were stained with 0.125 ug PE-Cy5 Anti-Hu/Mo CD11b .C57Bl/6 bone marrow cells were stained with 0.125 ug PE-Cy7 Anti-Hu/Mo CD11b  .C57Bl/6 bone marrow cells were stained with 0.25 ug Qfluor™ 710 Anti-Hu/Mo CD11b (QAB22) (solid line) or 0.25 ug Qfluor™ 710 Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 bone marrow cells were stained with 0.25 ug V450 Anti-Hu/Mo CD11b  (solid line) or 0.25 ug V450 Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 bone marrow cells were stained with 0.125 ug APC-Cy7 Anti-Hu/Mo CD11b  .C57Bl/6 bone marrow cells were stained with 0.125 ug Biotin Anti-Hu/Mo CD11b (QAB22) (solid line) or no primary antibody (dashed line). Flow Cytometry Data from 10,000 events., followed by Streptavidin FITC.C57Bl/6 bone marrow cells were stained with 0.5 ug FITC Anti-Hu/Mo CD11b (QAB22) (solid line) or 0.5 ug FITC Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 bone marrow cells were stained with 0.25 ug PerCP-Cy5.5 Anti-Hu/Mo CD11b .C57Bl/6 bone marrow cells were stained with 0.125 ug Anti-Hu/Mo CD11b PE (QAB22) (solid line) or 0.125 ug Rat IgG2b PE isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 bone marrow cells were stained with 0.125 ug PE-Cy5 Anti-Hu/Mo CD11b .C57Bl/6 bone marrow cells were stained with 0.125 ug PE-Cy7 Anti-Hu/Mo CD11b  .C57Bl/6 bone marrow cells were stained with 0.25 ug Qfluor™ 710 Anti-Hu/Mo CD11b (QAB22) (solid line) or 0.25 ug Qfluor™ 710 Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 bone marrow cells were stained with 0.25 ug V450 Anti-Hu/Mo CD11b  (solid line) or 0.25 ug V450 Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Anti-Human/Mouse CD11b Antibody [M1/70]

$ 58.00$ 268.00
Please Select Product Options Below To View The Catalog Number.

SKU: QAB22
Species: Human and Mouse
Tested Applications: Flow Cytometry
Available Conjugates: APC ;Unconjugated ;APC-Cy7 ;Biotin ;FITC ;PerCP-Cy5.5 ;PE ;PE-Cy5 ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

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In Stock: 1-2 Day Lead Time.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QAB22-APC-25ug

Human and Mouse Anti-CD11b Antibody Product Attributes

Species: Human and Mouse
Cross Reactivity: baboon, chimpanzee, cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD11b Antibody Clone: M1/70
Clone M1/70 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD11b Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD11b antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow. Low, but measureable presence of CD11b could be seen in cells in the molecular layer in cerebellum, glial cells in the caudate nucleus, cerebral cortex and hippocampus and macrophages in lung. We were unable to detect CD11b in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Lefort CT, Rossaint J, Moser M, Petrich BG, Zarbock A, Monkley SJ, Critchley DR, Ginsberg MH, Fassler R, and Ley K. 2012. Blood. 119:4275-4282. (in vitro blocking)Grewal JS, Pilgrim MJ, Grewal S, Kasman L, Werner P, Bruorton ME, London SD, and London L. 2011. FASEB J. 25:1680-1696. (Immunofluorescence microscopy - frozen tissue)Kim W-K, Sun Y, Do H, Autissier P, Halpern EF, Piatak M, Lifson JD, Burdo TH, McGrath MS, and Williams K. 2010. J. Leukoc. Biol. 87: 557-567. (Flow Cytometry - Rhesus macaque)Roland CL, Dineen SP, Lynn KD, Sullivan LA, Dellinger MT, Sadegh L, Sullivan JP, Shames DS, and Brekken RA. 2009. Mol. Cancer Ther. 8:1761-1771. (Immunofluorescence microscopy - frozen tissue)Sorg H, Lorch B, Jaster R, Fitzner B, Ibrahim S, Holzhueter S, Nizze H, and Vollmar B. 2008. Am. J. Physio. Gastrointest. Liver Physiol. 295: G1274-1280. (Immunohistochemistry - formalin-fixed paraffin embedded tissue)Kim DD, Miwa T, Kimura Y, Schwendener RA, van Lookeren Campagne M, and Song W-C. 2008. Blood. 112:1109-1119. (in vivo blocking)Ou R, Zhang M, Huang L, Flavell RA, Koni PA, and Moskophidis D. 2008. J. Virol. 82:2952-2965. (Immunohistochemistry - OCT embedded frozen tissue)Nutt SL, Metcalf D, D’Amico A, Polli M, and Wu L. 2005. J. Exp. Med. 201:221-231. (Immunomagnetic bead depletion)Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, and Hill TJ. 1995. J. Histochem. Cytochem. 43:313-320. (Immunohistochemistry - frozen tissue, paraffin embedded tissue)Miller LJ, Schwarting R, and Springer TA. 1986. J. Immunol. 137:2891-2900. (Immunoprecipitation)

Limitations and Warranty

enQuire Bio's Anti-Human/Mouse CD11b Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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