Human Anti-CD11c Antibody Product Attributes
Species: Human
Cross Reactivity: baboon, chimpanzee, cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD11c Antibody Clone: 3.9
Clone 3.9 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD11c Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD11c antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD11c could be seen inhematopoietic cells in the bone marrow, lymphoid tissue in appendix, macrophages in lung and squamous epithelial cells in the tonsil. We were unable to detect CD11c in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Selected References
Robinson BA, Estep RD, Messaoudi I, Rogers KS, and Wong SW. 2012. J. Virol. 86:2197-2211. (Flow cytometry - rhesus macaque)Campillo-Gimenez L, Laforge M, Fay M, Brussel A, et. al. 2010. J. Virol. 84(4):1838-1846. (Flow cytometry - African green monkey, rhesus macaque)Sadhu C, Hendrickson L, Dick KO, Potter TG, and Staunton DE. 2008. J. Immunoassay Immunochem. 29(1):42-57. (in vitro blocking)Arndt S, Melle C, Mondal K, Klein G, von Eggeling F, and Bosserhoff A-K. 2007. J. Leukoc. Biol. 82:1466-1472. (Immunoprecipitation)McGreal EP, Ikewaki N, Akatsu H, Morgan BP, and Gasque P. 2002. 168:5222-5232. (Immunofluorescence microscopy - frozen tissue)
Limitations and Warranty
enQuire Bio's Human Anti-CD11c Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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