C57Bl/6 splenocytes were stained with FITC Mouse Anti-MHC Class II  and 0.125 ug APC Mouse Anti-CD11c (QAB23) (right panel) or 0.125 ug APC Armenian Hamster IgG (left panel).

Mouse Anti-CD11c Antibody [N418]

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Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: Biotin ;Unconjugated ;APC ;APC-Cy7 ;FITC ;PerCP-Cy5.5 ;PE ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Armenian Hamster IgG
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB23-B-25ug
CD11c General Information
Alternate Names
Integrin alpha X, ITGAX
Curated Database and Bioinformatic Data
Gene SymbolItgax
Entrez Gene ID16411
Ensemble Gene IDENSMUSG00000030789
RefSeq Protein Accession(s)NP_067309
RefSeq mRNA Accession(s)NM_021334, XR_378219
RefSeq Genomic Accession(s)NC_000073
UniProt ID(s)Q9QXH4
UniGene ID(s)Q9QXH4
Cosmic ID(s)Itgax
KEGG Gene ID(s)mmu:16411
General Description of CD11c.
The N418 antibody reacts with mouse CD11c, also known as integrin alpha X. This 150 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha X (CD11c) assembles with Integrin beta-2 (CD18) into a receptor complex known as CR4 which can bind and induce signaling through ICAMs and VCAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.The N418 antibody is widely used as a marker for CD11c expression on dendritic cells (DC), often in parallel with markers for CD11b, for identification of developmental stages and mature subsets of this cell type. CD11c is prominently expressed on tissue macrophages, and is also detected on some types of activated T cells and intestinal intraepithelial lymphocytes (IEL).

Mouse Anti-CD11c Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD11c Antibody Clone: N418
Clone N418 Host and Isotype: Armenian Hamster IgG
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD11c Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD11c antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD11c could be seen inhematopoietic cells in the bone marrow, lymphoid tissue in appendix, macrophages in lung and squamous epithelial cells in the tonsil. We were unable to detect CD11c in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Guerriero JL, Ditsworth D, Catanzaro JM, Sabino G, Furie MB, Kew RR, Crawford HC, and Zong W-X. 2011. J. Immunol. 186: 3517-3526. (Immunohistochemistry - paraffin embedded tissue)Grewal JS, Pilgrim MJ, Grewal S, Kasman L, Werner P, Bruorton ME, London SD, and London L. 2011. FASEB J. 25:1680-1696. (Immunofluorescence microscopy - frozen tissue)Sadhu C, Ting HJ, Lipsky B, Hensley K, Garcia-Martinez LF, Simon SI, and Staunton DE. 2007. J. Leukoc. Biol. 81: 1395-1403. (in vitro blocking)Hagnerud, S, Manna PP, Cella M, Stenberg A, Frazier WA, Colonna M, and Oldenborg P-A. 2006. J. Immunol. 5772-5778. (Immunofluorescence microscopy - frozen tissue)Finkelman FD, Lees A, Birnbaum R, Gause WC, and Morris SC. 1996. J. Immunol. 157: 1406-1414. (in vivo activation)Huleatt JW and Lefrancois L. 1995. J. Immunol. 154: 5684-5693. (Immunoprecipitation)Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, and Steinman RM. 1990. J. Exp. Med. 171: 1753. (Immunoprecipitation)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD11c Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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