C57Bl/6 splenocytes were stained with FITC Mouse Anti-CD3  and 0.25 ug APC Mouse Anti-CD127 (QAB55) (right panel) or 0.25 ug APC Rat IgG2a isotype control (left panel).
C57Bl/6 splenocytes were stained with FITC Mouse Anti-CD3  and 0.25 ug APC Mouse Anti-CD127 (QAB55) (right panel) or 0.25 ug APC Rat IgG2a isotype control (left panel).C57Bl/6 splenocytes were stained with APC Mouse Anti-CD3  and 1 ug FITC Mouse Anti-CD127 (QAB55) (right panel) or 1 ug FITC Rat IgG2a isotype control (left panel).C57Bl/6 splenocytes were stained with FITC Mouse Anti-CD3 and 0.25 ug PerCP-Cy5.5 Mouse Anti-CD127 .C57Bl/6 splenocytes were stained with APC Mouse Anti-CD3 and 0.25 ug PE Mouse Anti-CD127 (QAB55) (right panel) or 0.25 ug PE Rat IgG2a isotype control (left panel).C57Bl/6 splenocytes were stained with FITC Mouse Anti-CD3  and 0.5 ug PE-Cy7 Mouse Anti-CD127 .C57Bl/6 splenocytes were stained with FITC Mouse Anti-CD3  and 0.5 ug V450 Mouse Anti-CD127  (right panel) or 0.5 ug V450 Rat IgG2a isotype control (left panel).

Anti-CD127 / IL-7Ra Antibody [A7R34]

$ 48.00$ 278.00
Please Select Product Options Below To View The Catalog Number.

SKU: QAB55
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: Biotin ;Unconjugated ;APC ;FITC ;PerCP-Cy5.5 ;PE ;PE-Cy7 ;V450
Isotype: Rat IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB55-B-25ug
CD127 / IL7R / IL7RA General Information
Alternate Names
IL7R, IL7RA, CDW127, CD127, ILRA, IL-7R-alpha, IL7RA
Curated Database and Bioinformatic Data
Gene SymbolIl17ra
Entrez Gene ID16172
Ensemble Gene IDENSMUSG00000002897
RefSeq Protein Accession(s)XP_006505681, NP_032385
RefSeq mRNA Accession(s)XM_006505618, NM_008359
RefSeq Genomic Accession(s)NC_000072, NG_039382
UniProt ID(s)Q60943
UniGene ID(s)Q60943
Cosmic ID(s)Il17ra
KEGG Gene ID(s)mmu:16172
General Description of CD127 / IL7R / IL7RA.
The A7R34 antibody is specific for mouse CD127, a 60-90 kDa cell surface protein also known as the Interleukin-7 Receptor alpha chain, or IL-7R alpha. CD127 is typically expressed at the cell surface as a heterodimer with the common gamma chain (CD132). This complex acts as the functional receptor for IL-7, a cytokine important in T and B cell development, and in mature T cell homeostasis. A second cytokine known as Thymic Stromal Lymphopoietin (TSLP) also binds to a receptor complex of CD127 and the TSLPR chain to trigger activation of dendritic cells, and is involved in B cell development, allergy and autoimmunity.The A7R34 antibody may be used as a phenotypic marker for CD127 on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8- ) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells. CD127 is a key marker, when used in combination with CD4 and CD25, to distinguish Treg and effector/memory Treg populations known as T(REM).

Mouse Anti-CD127 / IL7R / IL7RA Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD127 / IL7R / IL7RA Antibody Clone: A7R34
Clone A7R34 Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD127 / IL7R / IL7RA Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD127 / IL7R / IL7RA antibody reveals CD127 / IL7R / IL7RA expression is expected to be primarily localized to the cytosol and nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in CD127 / IL7R / IL7RA antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in exocrine glandular cells in the pancreas, glandular cells in the thyroid gland and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in exocrine glandular cells in the pancreas, glandular cells in the thyroid gland and non-germinal center cells in the tonsil. Low, but measureable presence of CD127 / IL7R / IL7RA could be seen in cells in the tubules in kidney, epidermal cells in the skin, glandular cells in the endometrium, fallopian tube, gallbladder, prostate and stomach, hepatocytes in liver, Leydig cells in the testis, myocytes in skeletal muscle, neuronal cells in the caudate nucleus and cerebral cortex and squamous epithelial cells in the esophagus. We were unable to detect CD127 / IL7R / IL7RA in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD127 / IL7R / IL7RA expression as measured by anti-CD127 / IL7R / IL7RA antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - + - + - - - - - - - - - - - - - - + -
IL17RA Variability + ++ + ++ ++ + + ++ + + + ++ ++ + + + ++ + ++ +

Selected References

Thaventhiran JED, Hoffmann A, Magiera L, de la Roche M, Lingel H, Brunner-Weinzierl M, and Fearon DT. 2012. Proc. Natl. Acad. Sci. 10.1073. (Flow cytometry).Jin J, Goldschneider I, and Lai L. 2011. J. Immunol. 186: 1915-1922. (in vivo activation)Vondenhoff MF, Greuter M, Goverse G, Elewaut D, Dewint P, Ware CF, Hoorweg K, Kraal G, and Mebius RE. 2009. J. Immunol. 182(9): 5439-5445. (Immunofluorescence microscopy - frozen tissue)Leithauser F, Meinhardt-Krajina T, Fink K, Wotschke B, Moller P and Reimann J. 2006. Am. J. Pathol. 168(6): 1898-1909. (Immunohistochemistry - frozen tissue)Seddon B and Zamoyska R. 2002. J. Immunol. 169: 2997-3005. (in vivo activation)Sudo T, Nishikawa S, Ohno N, Akiyama N, Tamakoshi M, Yoshida H and Nishikawa S-I. 1993. Proc. Natl. Acad. Sci. 90: 9125-9129. (in vitro and in vivo blocking; Immunoprecipitation)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD127 / IL-7Ra Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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