Human Anti-CD14 Antibody Product Attributes
Tested Applications: Flow Cytometry.
Application Notes: 5 µl per test where one test represents staining of a cell sample in a final volume of approximately 100 µL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD14 Antibody Clone: 61D3
Clone 61D3 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2
Antibody Concentration: 5 µL/test
Storage Conditions: 2-8°C protected from light. Stable for 12 Months. Do Not Freeze Conjugated Formats.
CD14 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:Staining with anti-CD14 antibody reveals CD14 expression is expected to be primarily localized to the plasma membrane and vesicles.
Observed Antibody Staining Data By Tissue Type:Variations in CD14 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in endothelial cells in the colon. More moderate antibody staining intensity was present in endothelial cells in the colon. Low, but measureable presence of CD14 could be seen in cells in the seminiferous ducts in testis, fibroblasts in skin, glandular cells in the fallopian tube and salivary gland, Langerhans in skin and Leydig cells in the testis. We were unable to detect CD14 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of CD14 expression as measured by anti-CD14 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Hogg N, Horton MA. 1987. In: McMichael AJ, Beverley PCL, Cobbold S, et al., eds. Leucocyte Typing III: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 576-602. Haziot A, Chen S, Ferrero E, Low MG, Silber R, Goyert SM. 1988. J Immunol. 141:547-552.
Limitations and Warranty
enQuire Bio's Human Anti-CD14 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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