Human Anti-CD147 / EMMPRIN / Neurothelin Antibody Product Attributes
Species: Human
Tested Applications: Bioactive, Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Inhibits Homotypic Aggregation, Adhesion to Matrix Proteins,, Migation through Matrigel, Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1:100-1:200), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD147 / EMMPRIN / Neurothelin Antibody Clone: 8D6
Clone 8D6 Host and Isotype: Mouse IgG1
Anti-Human CD147 / EMMPRIN / Neurothelin Positive Control Sample: HSB2 cells. Renal Cell, Ovarian carcinoma or Melanoma
Cellular Localization of Antibody <8D6 Staining: Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human T cell line Molt 13
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
CD147 / EMMPRIN / Neurothelin Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-BSG antibody staining is expected to be primarily localized to the vesicles.Observed Antibody Staining Data By Tissue Type:
Variations in CD147 / EMMPRIN / Neurothelin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the seminiferous ducts in testis, cells in the tubules in kidney, glandular cells in the colon, epididymis, rectum and stomach and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in cells in the seminiferous ducts in testis, cells in the tubules in kidney, glandular cells in the colon, epididymis, rectum and stomach and trophoblastic cells in the placenta. Low, but measureable presence of CD147 / EMMPRIN / Neurothelin could be seen inepidermal cells in the skin, exocrine glandular cells in the pancreas, glandular cells in the adrenal gland and parathyroid gland, squamous epithelial cells in the cervix and uterine, tonsil and vagina. We were unable to detect CD147 / EMMPRIN / Neurothelin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of CD147 / EMMPRIN / Neurothelin expression as measured by anti-CD147 / EMMPRIN / Neurothelin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | - | - | ++ | ++ | ++ | ++ | ++ | ++ | ++ | + | + | ++ | + | + | ++ | + | + | ++ | ++ | ++ |
| BSG Variability | ++ | ++ | ++ | +++ | +++ | ++ | ++ | +++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
Limitations and Warranty
enQuire Bio's CD147 / EMMPRIN / Neurothelin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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