Human Anti-CD15 / FUT4 Antibody Product Attributes
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Anti-CD15 / FUT4 Antibody Clone: FUT4/815 + BRA-4F1
Clone FUT4/815 + BRA-4F1 Host and Isotype: Mouse IgM
Anti-Human CD15 / FUT4 Positive Control Sample: U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma
Cellular Localization of Antibody Cell surface, granular paranuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Purified human neutrophils (FUT4/815); Myelomonocytic leukemia cells (BRA-4F1)
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
CD15 / FUT4 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-FUT4 antibody staining is expected to be primarily localized to the vesicles.
Observed Antibody Staining Data By Tissue Type:
Variations in CD15 / FUT4 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow. Low, but measureable presence of CD15 / FUT4 could be seen in cells in the red pulp in spleen, glandular cells in the stomach and non-germinal center cells in the lymph node and tonsil. We were unable to detect CD15 / FUT4 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's CD15 / FUT4 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.