FACS analysis of human Monocytes using CD15 Monoclonal Antibody (Leu-M1).
FACS analysis of human Monocytes using CD15 Monoclonal Antibody (Leu-M1).

Anti-CD15 / FUT4 Antibody Clone Leu-M1; same as MMA

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 2526-MSM1
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgM kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 2526-MSM1-P1
CD15 / FUT4 General Information
Alternate Names
FUT4, Fucosyltransferase 4, Myeloid-Specific alpha (1,3) Fucosyltransferase
Molecular Weight
~220kDa
Chromosomal Location
11q21
Curated Database and Bioinformatic Data
Gene SymbolFUT4
Entrez Gene ID2526
Ensemble Gene IDENSG00000196371
RefSeq Protein Accession(s)NP_002024
RefSeq mRNA Accession(s)NM_002033
RefSeq Genomic Accession(s)NC_000011, NC_018922
UniProt ID(s)P22083
UniGene ID(s)P22083
HGNC ID(s)4015
Cosmic ID(s)FUT4
KEGG Gene ID(s)hsa:2526
PharmGKB ID(s)PA28431
General Description of CD15 / FUT4.
CD15 plays a role in mediating phagocytosis, bactericidal activity,, chemotaxis. It is present on >95% of granulocytes including neutrophils, eosinophils, to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells, some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B, T phenotypes which otherwise have a quite distinct histological appearance.

Human Anti-CD15 / FUT4 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD15 / FUT4 Antibody Clone: Leu-M1; same as MMA
Clone Leu-M1; same as MMA Host and Isotype: Mouse IgM kappa
Anti-Human CD15 / FUT4 Positive Control Sample: U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma
Cellular Localization of Antibody Cell surface, granular paranuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: U937 histiocytic cell line
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD15 / FUT4 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-FUT4 antibody staining is expected to be primarily localized to the vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in CD15 / FUT4 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow. Low, but measureable presence of CD15 / FUT4 could be seen in cells in the red pulp in spleen, glandular cells in the stomach and non-germinal center cells in the lymph node and tonsil. We were unable to detect CD15 / FUT4 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's CD15 / FUT4 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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