baboon, cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-Anti-CD152 Antibody Clone: BNI3
Clone BNI3 Host and Isotype: Mouse IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
Anti-CD152 Previously Observed Antibody Staining Patterns
Moreno-Fernandez ME, Rueda CM, Rusie LK, and Chougnet CA. 2011. Blood. 117: 5372-5380. (in vitro blocking)Schonfeld D, Matschiner G, Chatwell L, Trentmann S, Gille H, Hulsmeyer M, Brown N, Kaye PM, Schlehuber S, Hohlbaum AM and Skerra A. 2009. 106: 8198-8203. (Immunohistochemistry - frozen tissue)Rivas MN, Weatherly K, Hazzan M, Vokaer B, Dremier S, Gaudray F, Goldman M, Salmon I, and Braun MY. 2009. 183:4284-4291. (in vitro blocking)Bonzheim I, Geissinger E, Tinguely M, Roth S, Grieb T, Reimer P, Wilhelm M, Rosenwald A, Muller-Hermelink HK, and Rudiger T. 2008. Am. J. Clin. Pathol. 130: 613-619. (Immunohistochemistry - paraffin embedded tissue; Immunofluorescence microscopy - frozen tissue)Young NT, Waller ECP, Patel R, Roghanian A, Austyn JM, and Trowsdale J. 2008. 111: 3090-3096. (in vitro activation)Wei B, da Rocha Dias S, Wang H and Rudd CE. 2007. J. Immunol. 179: 400-408. (in vitro activation)Jonuleit H, Schmitt E, Stassen M, Tuettenberg A, Knop J and Enk AH. 2001. J. Exp. Med. 193: 1285-1294 (in vitro blocking)Oaks MK and Hallett KM. 2000. J. Immunol. 164: 5015-5018. (Immunoprecipitation; EIA - plate coating)
Limitations and Warranty
enQuire Bio's Human Anti-CD152 / CTLA-4 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.