Human Anti-CD161 Antibody Product Attributes
Cross Reactivity: chimpanzee, baboon, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD161 Antibody Clone: HP-3G10
Clone HP-3G10 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD161 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in CD161 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen, cells in the tubules in kidney, fibroblasts in skin, glandular cells in the epididymis and prostate, hematopoietic cells in the bone marrow and myocytes in heart muscle. More moderate antibody staining intensity was present in cells in the red pulp in spleen, cells in the tubules in kidney, fibroblasts in skin, glandular cells in the epididymis and prostate, hematopoietic cells in the bone marrow and myocytes in heart muscle. Low, but measureable presence of CD161 could be seen in cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, cells in the white pulp in spleen, endothelial cells in the colon, fibroblasts in mesenchymal tissue, germinal center cells in the tonsil, glandular cells in the cervix, uterine, endometrium, salivary gland and thyroid gland, glial cells in the hippocampus, lymphoid tissue in appendix, neuronal cells in the hippocampus, neuropil in cerebral cortex, peripheral nerve in mesenchymal tissue, peripheral nerve/ganglion in colon, pneumocytes in lung and respiratory epithelial cells in the nasopharynx. We were unable to detect CD161 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of CD161 expression as measured by anti-CD161 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Yamada H, Nakashima Y, Okazaki K, Mawatari T, Fukushi J-I, Oyamada A, Fujimura K, Iwamoto Y, and Yoshikai Y. 2011. J. Rheumatol. 38: 1569-1575. (Flow cytometry)Fogal B, Yi T, Wang C, Rao DA, Lebastchi A, Kulkarni S, Tellides G, and Pober JS. 2011. J. Immunol. 187: 6268-6280. (in vitro depletion)Pozo, D, Vales-Gomez, Mavaddat N, Williamson SC, Chisholm SE, and Reyburn H. 2006. J. Immunol. 176: 2397-2406. (Western blot)Exley M, Porcelli S, Furman M, Garcia J, and Balk S. 1998. J. Exp. Med. 188: 867-876. (in vitro blocking, Western blot)
Limitations and Warranty
enQuire Bio's Human Anti-CD161 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.