Immunohistochemical staining of CD163  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

CD163 [10D6] Antibody (cGMP).

$ 128.00$ 728.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD91
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1

Product NumberDescriptionPrice
QIVD91-0.1ml Size: 0.1 ml, Format: Concentrate $ 128.00
QIVD91-0.5ml Size: 0.5 ml, Format: Concentrate $ 428.00
QIVD91-1ml Size: 1 ml, Format: Concentrate $ 728.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
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SKU: QIVD91-0.1ml
CD163 General Information
Alternate Names
Molecular Weight
125.5 kDa
Chromosomal Location
p13.31 [chr: 12] [chr_start: 7470813] [chr_end: 7503893] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolCD163
Entrez Gene ID9332
RefSeq Protein Accession(s)XP_016875720; NP_981961; NP_004235
RefSeq mRNA Accession(s)XR_002957389; NM_004244; NM_203416; XM_024449278; NM_001370146; NM_001370145; NR_163255
RefSeq Genomic Accession(s)NG_029826; NC_000012
UniProt ID(s)Q86VB7
PharmGKB ID(s)PA26190
KEGG Gene ID(s)hsa:9332
General Description of CD163 .
The CD163 molecule is a type I membrane protein also known as M130 antigen, Ber-Mac3, Ki-M8 or SM4. CD163 protein is restricted in its expression to the monocytic/macrophage lineage. It is reported to be present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhans cells. In addition, multinucleated cells within inflammatory lesions are reported not to express CD163 protein. The protein is upregulated by glucocorticoids and downregulated by the immunosuppressant cyclosporin A and by phorbol esters.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-CD163 Antibody Clone: 10D6
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody 10D6 Staining: Membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Human CD163 antigen.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

CD163 Information for Pathologists

Summary:

CD163 is a scavenger receptor that binds hemoglobin-haptoglobin complexes in plasma. The soluble form of the protein attenuates immune responses.

Common Uses By Pathologists:

CD163 staining is observed in histiocytic sarcoma. Monocytes and macrophages stain CD163+ in most healthy and disease samples.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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CD163 [10D6] Antibody (cGMP).