Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD19 Antibody [HIB19]

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Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: PE ;APC ;FITC ;PerCP-Cy5.5 ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB30-PE-25Tests
CD19 General Information
Alternate Names
CD19, Leu-12, B4, CVID3
Curated Database and Bioinformatic Data
Gene SymbolCD19
Entrez Gene ID930
Ensemble Gene IDENSG00000177455
RefSeq Protein Accession(s)XP_011544283, NP_001761, XP_006721166, XP_016879382, NP_001171569
RefSeq mRNA Accession(s)XM_017023893, NM_001770, XM_006721103, XM_011545981, XR_950871 NM_001178098
RefSeq Genomic Accession(s)NC_018927, NG_007275, NC_000016
UniProt ID(s)P15391
UniGene ID(s)P15391
HGNC ID(s)1633
Cosmic ID(s)CD19
KEGG Gene ID(s)hsa:930
PharmGKB ID(s)PA26192
General Description of CD19.
The HIB19 antibody reacts with human CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21, CD81 and CD225, in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells.The HIB19 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.

Human Anti-CD19 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD19 Antibody Clone: HIB19
Clone HIB19 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD19 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD19 / Leu-12 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the lymph node and tonsil, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the lymph node and tonsil, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD19 / Leu-12 could be seen in. We were unable to detect CD19 / Leu-12 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Kroenke MA, Eto D, Locci M, Cho M, Davidson T, Haddad EK, and Crotty S. 2012. J. Immunol. 188: 3734-3744. (Flow cytometry)So NSY, Ostrowski MA, and Gray-Owen SD. 2012. J. Immunol. 188: 4008-4022. (in vitro cell capture for microscopy)Zhang L, Yang N, Conejo-Garcia J-R, Katsaros D, Mohamed-Hadley A, Fracchioli S, Schlienger K, Toll A, Levine B, Rubin SC, and Coukos G. 2003. Clin. Cancer Res. 9: 264 - 272. (Immunohistochemistry)Hibe W, Dirnhofer S, Oberwasserlechner F, Eisterer W, Amman K, Schmid T, Hilbe G, Thaler J, and Woll E. 2003. J. Clin. Pathol. 56: 736-741. (Immunohistochemistry - frozen tissues)

Limitations and Warranty

enQuire Bio's Human Anti-CD19 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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