C57Bl/6 splenocytes were stained with 0.125 ug APC Mouse Anti-CD19 (QAB28) (solid line) or 0.125 ug APC Rat IgG2a isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Mouse Anti-CD19 Antibody [1D3]

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Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: Biotin ;Unconjugated ;APC ;FITC ;PerCP-Cy5.5 ;PE ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Rat IgG2a kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB28-B-25ug
CD19 / Leu-12 General Information
Alternate Names
CD19, Leu-12, B4, CVID3
Curated Database and Bioinformatic Data
Gene SymbolCd19
Entrez Gene ID12478
Ensemble Gene IDENSMUSG00000030724
RefSeq Protein Accession(s)XP_006507346, XP_006507345, NP_033974
RefSeq mRNA Accession(s)XM_006507282, XM_006507283, NM_009844
RefSeq Genomic Accession(s)NC_000073
UniProt ID(s)P25918, Q3TQG5, A0A0X1KG58
UniGene ID(s)P25918, Q3TQG5, A0A0X1KG58
Cosmic ID(s)Cd19
KEGG Gene ID(s)mmu:12478
General Description of CD19 / Leu-12.
The 1D3 antibody reacts with mouse CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21 and CD81, in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells. The 1D3 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.

Mouse Anti-CD19 / Leu-12 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD19 / Leu-12 Antibody Clone: 1D3
Clone 1D3 Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD19 / Leu-12 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD19 / Leu-12 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the lymph node and tonsil, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the lymph node and tonsil, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD19 / Leu-12 could be seen in. We were unable to detect CD19 / Leu-12 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Ghosn EEB, Yamamoto R, Hamanada S, Yang Y, Herzenberg LA, Nakauchi H, and Herzenberg LA. 2012. Proc. Natl. Acad. Sci. 109:5394-5398. (Flow cytometry)Raghavan S, Ostberg AK, Flach C-F, Ekman A, Blomquist M, Czerkinsky C, and Holmgren J. 2010. Infect. Immun. 78(10)4251-4260. (Immunohistochemistry - acetone fixed tissue)Togayachi A, Kozono Y, Ikehara Y, Ito H, et al. 2010. Proc. Natl. Acad. Sci. 107:11900-11905. (Immunoprecipitation)Poitrasson-Riviere M, Bienvenu B, Le Campion A, Becourt C, Martin B, and Lucas B. 2008. J. Immunol. 180:7294-7304. (Immunohistochemistry - paraffin embedded tissue)Lee Y, Haas KM, Gor DO, Ding X, Karp DR, Greenspan NS, Poe JC, and Tedder TF. 2005. J. Immunol. 175:8011-8023. (Immunoprecipitation)Bobbitt KR and Justement LB. 2000. J. Immunol. 165: 5588-5596. (in vitro stimulation, Immunoprecipitation)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD19 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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