Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.
Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug FITC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug PerCPMouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  .Human PBMCs were stained with 5 uL  .

Anti-CD19 Antibody [SJ25C1]

$ 68.00$ 1,518.00
Please Select Product Options Below To View The Catalog Number.

SKU: QAB29
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: PE ;APC ;FITC ;PerCP ;PE-Cy5 ;PE-Cy7
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
QAB29-PE-25Tests Size: 25 tests, Tag: PE $ 68.00
QAB29-APC-100Tests Size: 100 tests, Tag: APC $ 228.00
QAB29-APC-25Tests Size: 25 tests, Tag: APC $ 108.00
QAB29-APC-500Tests Size: 500 tests, Tag: APC $ 998.00
QAB29-F-100Tests Size: 100 tests, Tag: FITC $ 208.00
QAB29-F-25Tests Size: 25 tests, Tag: FITC $ 98.00
QAB29-F-500Tests Size: 500 tests, Tag: FITC $ 908.00
QAB29-PCP-100Tests Size: 100 tests, Tag: PerCP $ 318.00
QAB29-PCP-25Tests Size: 25 tests, Tag: PerCP $ 128.00
QAB29-PCP-500Tests Size: 500 tests, Tag: PerCP $ 1,388.00
QAB29-PE-100Tests Size: 100 tests, Tag: PE $ 158.00
QAB29-PE-500Tests Size: 500 tests, Tag: PE $ 658.00
QAB29-PE5-100Tests Size: 100 tests, Tag: PE-Cy5 $ 338.00
QAB29-PE5-25Tests Size: 25 tests, Tag: PE-Cy5 $ 148.00
QAB29-PE5-500Tests Size: 500 tests, Tag: PE-Cy5 $ 1,498.00
QAB29-PE7-100Tests Size: 100 tests, Tag: PE-Cy7 $ 348.00
QAB29-PE7-25Tests Size: 25 tests, Tag: PE-Cy7 $ 158.00
QAB29-PE7-500Tests Size: 500 tests, Tag: PE-Cy7 $ 1,518.00
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QAB29-PE-25Tests
CD19 / Leu-12 General Information
Alternate Names
CD19, Leu-12, B4, CVID3
Curated Database and Bioinformatic Data
Gene SymbolCD19
Entrez Gene ID930
Ensemble Gene IDENSG00000177455
RefSeq Protein Accession(s)XP_011544283, NP_001761, XP_006721166, XP_016879382, NP_001171569
RefSeq mRNA Accession(s)XM_017023893, NM_001770, XM_006721103, XM_011545981, XR_950871 NM_001178098
RefSeq Genomic Accession(s)NC_018927, NG_007275, NC_000016
UniProt ID(s)P15391
UniGene ID(s)P15391
HGNC ID(s)1633
Cosmic ID(s)CD19
KEGG Gene ID(s)hsa:930
PharmGKB ID(s)PA26192
General Description of CD19 / Leu-12.
The SJ25C1 antibody reacts with human CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21 (CR2), CD81 (TAPA-1) and CD225 (Leu13), in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. Upon activation and tyrosine phosphorylation, the CD19 molecule can provide an anchor for cytoplasmic signaling proteins such as GRB2, SOS or PLCG2. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells.The SJ25C1 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.

Human Anti-CD19 / Leu-12 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD19 / Leu-12 Antibody Clone: SJ25C1
Clone SJ25C1 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD19 / Leu-12 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD19 / Leu-12 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the lymph node and tonsil, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the lymph node and tonsil, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD19 / Leu-12 could be seen in. We were unable to detect CD19 / Leu-12 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Piatosa B, Birbach M, Siewiera K, Ussowicz M, Kalwak K, Drabko K, Rekawek A, Tkaczyk K, Kurowski PN. 2013. Cytometry Part B. 84B: 179-186. (Flow Cytometry)Sekiguchi DR, Smith SB, Sutter JA, Goodman NG, Propert K, Louzoun Y, Rogers W, Luning Prak ET. 2011. Cytometry Part B. 80B: 291-299. (Flow Cytometry)Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. B-cell antigens: CD19. In: Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:34-36. (Flow Cytometry)Karahan GE, Eikmans M, Anholts JDH, Class FHJ and Heidt S. 2014. Clin Exp Immunol. 177(1): 333-340. (Flow Cytometry)Bansal RR, Mackay CR, Moser B and Ebert M. 2012. Eur J Immunol. 42(1): 110-119. (Flow Cytometry)Jourdan M, Caraux A, Caron G, Robert N, Fiol G, Rème T, Bolloré K, Vendrell JP, Le Gallou S, Mourcin F, De Vos J, Kassambara A, Duperray C, Hose D, Fest T, Tarte K and Klein B. 2011. J Immunol. 187(8): 3931-3941. (Flow Cytometry)Goval J-J, Greimers R, Boniver J and de Leval L. 2006. J Histochem Cytochem. 54(1): 75-84. (Immunofluorescence - frozen tissue)

Limitations and Warranty

enQuire Bio's Human Anti-CD19 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-CD19 Antibody [SJ25C1]”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Anti-CD19 Antibody [SJ25C1]

https://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB29-APC-100Tests-anti-CD19-Leu-12-antibody-10-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB29-F-100Tests-anti-CD19-Leu-12-antibody-10-300x296.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB29-PCP-100Tests-anti-CD19-Leu-12-antibody-10-300x139.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB29-PE-100Tests-anti-CD19-Leu-12-antibody-10-292x300.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB29-PE5-100Tests-anti-CD19-Leu-12-antibody-10-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB29-PE7-100Tests-anti-CD19-Leu-12-antibody-10-300x295.png