Discontinued
Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:50 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Clonality: Monoclonal
Anti-CD1a Antibody Clone: O10
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody O10 Staining: Cytoplasmic, cell membrane
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with thymus cells.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
CD1a Information for Pathologists
Summary:
CD1a is a T cell surface antigen found on subsets of normal T cells. It is important in antigen presentation to dendritic cells, particularly glycolipids and lipopeptide antigens that traffic through early endocytic pathways. CD1a is also referred to as Leu6.Common Uses By Pathologists:
CD1a immunostaining is used to diagnose Langerhans cell histiocytosis and to exclude other entities that are CD1a negative. It is also a useful means of identifying Langerhans cells in inflammatory or neoplastic disorders.Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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