Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD2 Antibody [RPA-2.10]

$ 99.00$ 1,639.00
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SKU: QAB1
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;APC ;PerCP ;PE ;V450
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB1-F-25Tests
CD2 / LY37 General Information
Alternate Names
CD2, LY37, OX-34, CD2R, LFA-2, LFA2, OX34
Curated Database and Bioinformatic Data
Gene SymbolCD2
Entrez Gene ID914
Ensemble Gene IDENSG00000116824
RefSeq Protein Accession(s)NP_001315538, NP_001758
RefSeq mRNA Accession(s)NM_001767, NM_001328609
RefSeq Genomic Accession(s)NC_000001, NC_018912, NG_050908
UniProt ID(s)Q53F96, P06729
UniGene ID(s)Q53F96, P06729
HGNC ID(s)1639
Cosmic ID(s)CD2
KEGG Gene ID(s)hsa:914
PharmGKB ID(s)PA26198
General Description of CD2 / LY37.
The RPA-2.10 antibody reacts with human CD2, an approximately 50 kDa glycoprotein, and a member of the Ig superfamily. CD2, also known as LFA-2, is a receptor for CD58 in the human and is expressed on the cell surface of 80-90% of human peripheral blood lymphocytes, a subset of NK cells, and all mature T cells. CD2 mediates lymphocyte adhesion and is involved in T cell activation.RPA-2.10 is reported to block mixed lymphocyte reaction. Please choose the appropriate format for each application.

Human Anti-CD2 / LY37 Antibody Product Attributes

Species: Human
Cross Reactivity: baboon, chimpanzee, cynomolgus monkey, capuchin monkey, rhesus macaque, pig
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD2 / LY37 Antibody Clone: RPA-2.10
Clone RPA-2.10 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD2 / LY37 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD2 / LY37 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the white pulp in spleen. More moderate antibody staining intensity was present in cells in the white pulp in spleen. Low, but measureable presence of CD2 / LY37 could be seen in. We were unable to detect CD2 / LY37 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD2 / LY37 expression as measured by anti-CD2 / LY37 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - + - - - - - - - - - - - - - - - +
CD2 Variability + ++ + ++ + + ++ + ++ ++ + + + + + + + ++ ++ +++

Selected References

Knapp W, Dorken B, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.Aversa GG, Bishop GA, Suranyi MG and Hall BM. 1987. Transplant Proc. 19: 277-278.Hahn WC, Burakoff SJ, Bierer BE. 1993. J Immunol. 150(7):2607-2619.Xu H, Elster EA, Blair PJ, Burkly LC, Tadaki DK, Harlan DM and Kirk AD. 2003. Am J Transplant. 3: 1350-1354. (Immunohistochemistry - frozen tissue)Kim EO, Kim TJ, Kim N, Kim ST, Kumar V and Lee KM. 2010. J Biol Chem. 285: 41755-41764. (in vitro blocking)Schernthaner GH, Jordan JH, Ghannadan M, Agis H, Bevec D, Nunez R, Escribano L, Majdic O, Willheim M, Worda C, Printz D, Fritsch G, Lechner K and Valent P. 2001. Blood. 98(13): 3784-3792. (Flow Cytometry)Herodin F, Thullier P, Garin D and Drouet M. 2005. Eur Cytokine Netw. 16(2): 104-116. (Flow Cytometry - Baboon, Chimpanzee, Cynomolgus, Rhesus)

Limitations and Warranty

enQuire Bio's Human Anti-CD2 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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