Human Anti-CD2 / LY37 Antibody Product Attributes
Cross Reactivity: baboon, chimpanzee, cynomolgus monkey, capuchin monkey, rhesus macaque, pig
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD2 / LY37 Antibody Clone: RPA-2.10
Clone RPA-2.10 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD2 / LY37 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in CD2 / LY37 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the white pulp in spleen. More moderate antibody staining intensity was present in cells in the white pulp in spleen. Low, but measureable presence of CD2 / LY37 could be seen in. We were unable to detect CD2 / LY37 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of CD2 / LY37 expression as measured by anti-CD2 / LY37 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Knapp W, Dorken B, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.Aversa GG, Bishop GA, Suranyi MG and Hall BM. 1987. Transplant Proc. 19: 277-278.Hahn WC, Burakoff SJ, Bierer BE. 1993. J Immunol. 150(7):2607-2619.Xu H, Elster EA, Blair PJ, Burkly LC, Tadaki DK, Harlan DM and Kirk AD. 2003. Am J Transplant. 3: 1350-1354. (Immunohistochemistry - frozen tissue)Kim EO, Kim TJ, Kim N, Kim ST, Kumar V and Lee KM. 2010. J Biol Chem. 285: 41755-41764. (in vitro blocking)Schernthaner GH, Jordan JH, Ghannadan M, Agis H, Bevec D, Nunez R, Escribano L, Majdic O, Willheim M, Worda C, Printz D, Fritsch G, Lechner K and Valent P. 2001. Blood. 98(13): 3784-3792. (Flow Cytometry)Herodin F, Thullier P, Garin D and Drouet M. 2005. Eur Cytokine Netw. 16(2): 104-116. (Flow Cytometry - Baboon, Chimpanzee, Cynomolgus, Rhesus)
Limitations and Warranty
enQuire Bio's Human Anti-CD2 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.