enQuire Bio » Products » Antibody » Anti-CD22 / BL-CAM Antibody Clone BLCAM/1795
Western Blot Analysis of Raji and Ramos Cell Lysates using CD22 Monoclonal Antibody (BLCAM/1795).
Western Blot Analysis of Raji and Ramos Cell Lysates using CD22 Monoclonal Antibody (BLCAM/1795).

Anti-CD22 / BL-CAM Antibody Clone BLCAM/1795

$ 199.00 – $ 459.00
Please Select Product Options Below To View The Catalog Number.

SKU: 933-MSM5
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
933-MSM5-P0 Size: 20 ug, Tag: Unconjugated $ 199.00
933-MSM5-P1 Size: 100 ug, Tag: Unconjugated $ 429.00
933-MSM5-P2 Size: 200 ug, Tag: Unconjugated $ 459.00
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In Stock.
Flat Rate Shipping Anywhere in the US: $45
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SKU: 933-MSM5-P0
CD22 / BL-CAM General Information
Alternate Names
CD22, Cluster of Differentiation 22
Molecular Weight
130-140kDa
Chromosomal Location
19q13.1
Curated Database and Bioinformatic Data
Gene SymbolCD22
Entrez Gene ID933
Ensemble Gene IDENSG00000012124
RefSeq Protein Accession(s)NP_001172028, NP_001172029, NP_001265346, NP_001762, NP_001172030
RefSeq mRNA Accession(s)NM_001185101, NM_001278417, NM_001185100, NM_001771, NM_024916, NM_001185099
RefSeq Genomic Accession(s)NC_018930, NC_000019,
UniProt ID(s)Q0EAF5, P20273
UniGene ID(s)Q0EAF5, P20273
HGNC ID(s)1643
Cosmic ID(s)CD22
KEGG Gene ID(s)hsa:933
PharmGKB ID(s)PA26201
General Description of CD22 / BL-CAM.
Recognizes a protein of 130-140kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD+. It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.

Human Anti-CD22 / BL-CAM Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-CD22 / BL-CAM Antibody Clone: BLCAM/1795
Clone BLCAM/1795 Host and Isotype: Mouse IgG1 kappa
Anti-Human CD22 / BL-CAM Positive Control Sample: Raji, Daudi, IM9, JY25 and human peripheral blood lymphocytes. Human Tonsil
Cellular Localization of Antibody Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human BL-CAM/CD22 protein (aa52-178)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD22 / BL-CAM Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD22 / BL-CAM antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the white pulp in spleen and germinal center cells in the tonsil. More moderate antibody staining intensity was present in cells in the white pulp in spleen and germinal center cells in the tonsil. Low, but measureable presence of CD22 / BL-CAM could be seen innon-germinal center cells in the lymph node. We were unable to detect CD22 / BL-CAM in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD22 / BL-CAM expression as measured by anti-CD22 / BL-CAM antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - + - - - - - - - - - -
CD22 Variability + + + + + + + + + ++ + + + + + + + + + +

Limitations and Warranty

enQuire Bio's CD22 / BL-CAM Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD22 / BL-CAM Antibody Clone BLCAM/1795

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