C57Bl/6 splenocytes were stimulated with anti-IgM and anti-CD40 for 4 days. Cells were then stained with 0.25 ug APC Mouse Anti-CD252 (QAB75) (solid line) or 0.25 ug APC Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Mouse Anti-CD252 / OX40-L Antibody [RM134L]

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SKU: QAB75
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: Biotin ;APC
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB75-B-25ug
CD252 / OX-40L / TNFSF4 General Information
Alternate Names
OX-4L, TNFSF4, CD252, GP34, OX-4L, TNLG2B, CD134L, CD252, TXGP1, OX4OL
Curated Database and Bioinformatic Data
Gene SymbolTnfsf4
Entrez Gene ID22164
Ensemble Gene IDENSMUSG00000026700
RefSeq Protein Accession(s)NP_033478
RefSeq mRNA Accession(s)NM_009452
RefSeq Genomic Accession(s)NC_000067
UniProt ID(s)B6DXE3, P43488
UniGene ID(s)B6DXE3, P43488
Cosmic ID(s)Tnfsf4
KEGG Gene ID(s)mmu:22164
General Description of CD252 / OX-40L / TNFSF4.
The RM134L antibody recognizes CD252, also known as OX-40 Ligand or CD134 Ligand, a member of the TNF superfamily that is present on the surface of antigen presenting cells and activated B lymphocytes. The OX-40 Ligand interacts with OX-40 (CD134) which is expressed primarily on activated T cells. This costimulatory interaction leads to increased proliferation and IL-2 production responses of activated T cells, and at the same time enhances proliferation and immunoglobulin secretion by activated B cells.The RM134L antibody is useful for flow cytometric detection of CD252 on stimulated mouse splenocytes. It has also been reported to block the costimulatory activity of OX-40 Ligand. Please choose the appropriate format for each application.

Mouse Anti-CD252 / OX-40L / TNFSF4 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD252 / OX-40L / TNFSF4 Antibody Clone: RM134L
Clone RM134L Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH 7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD252 / OX-40L / TNFSF4 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD252 / OX-40L / TNFSF4 antibody reveals CD252 / OX-40L / TNFSF4 expression is expected to be primarily localized to the nucleus and vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in CD252 / OX-40L / TNFSF4 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the appendix, colon and duodenum, squamous epithelial cells in the esophagus, myocytes in heart muscle, squamous epithelial cells in the oral mucosa, glandular cells in the rectum and salivary gland, epidermal cells in the skin, glandular cells in the small intestine, smooth muscle cells in the smooth muscle and cells in the seminiferous ducts in testis. More moderate antibody staining intensity was present in glandular cells in the appendix, colon and duodenum, squamous epithelial cells in the esophagus, myocytes in heart muscle, squamous epithelial cells in the oral mucosa, glandular cells in the rectum and salivary gland, epidermal cells in the skin, glandular cells in the small intestine, smooth muscle cells in the smooth muscle and cells in the seminiferous ducts in testis. Low, but measureable presence of CD252 / OX-40L / TNFSF4 could be seen inlymphoid tissue in appendix, hematopoietic cells in the bone marrow, myoepithelial cells in the breast, neuronal cells in the caudate nucleus, endothelial cells in the cerebral cortex, neuronal cells in the cerebral cortex, neuropil in cerebral cortex, endothelial cells in the colon, neuronal cells in the hippocampus, cells in the glomeruli in kidney, bile duct cells in the liver, macrophages in lung, pneumocytes in lung, germinal center cells in the lymph node, follicle cells in the ovary, exocrine glandular cells in the pancreas, glandular cells in the prostate and urothelial cells in the urinary bladder. We were unable to detect CD252 / OX-40L / TNFSF4 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD252 / OX-40L / TNFSF4 expression as measured by anti-CD252 / OX-40L / TNFSF4 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - + + + - ++ - - - - + ++ - - + - + -
TNFSF4 Variability ++ + + ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ +

Selected References

Akiba H, Oshima H, Takeda K, Atsuta M, Nakano H, Nakajima A, Nohara C, Yagita H and Okumura K. 1999 J Immunol. 162(12): 7058-7066. (Flow cytometry, in vitro blocking)Stuber E, Neurath M, Calderhead D, Fell HP and Strober W. 1995 Immunity. 2(5): 507-521.Sibilano R, Frossi B, Suzuki R, D'Incà F, Gri G, Piconese S, Colombo MP, Rivera J and Pucillo CE. 2012 Journal of Allergy Clin Immunol. 130(3): 751-760. (Blocking)van der Merwe M, Abdelsamed HA, Seth A, Ong T, Vogel P and Pillai AB. 2013 J Immunol. 191(11): 5764-5776 (Flow cytometry)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD252 / OX40-L Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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