Human PBMCs were stained with 5 uL  (solid line) or 0.25 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD28 Antibody [CD28.2]

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Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;APC ;PerCP-Cy5.5 ;PE ;PE-Cy7
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB37-F-25Tests
CD28 General Information
Alternate Names
CD28, TP44, CD28RNA
Curated Database and Bioinformatic Data
Gene SymbolCD28
Entrez Gene ID940
Ensemble Gene IDENSG00000178562
RefSeq Protein Accession(s)XP_011510497, XP_011510496, XP_011510499, NP_001230006, NP_001230007, NP_006130
RefSeq mRNA Accession(s)NM_001243078, XM_011512195, NM_001243077, XM_011512194, XM_011512197, NM_006139
RefSeq Genomic Accession(s)NC_018913, NG_029618, NC_000002
UniProt ID(s)P10747, B4E0L1
UniGene ID(s)P10747, B4E0L1
HGNC ID(s)1653
Cosmic ID(s)CD28
KEGG Gene ID(s)hsa:940
PharmGKB ID(s)PA26207
General Description of CD28.
The CD28.2 antibody reacts with human CD28, a 44 kDa type I surface glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), which are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of receptors include CTLA-4 (CD152), PD-1 (CD279), ICOS and BTLA.The CD28.2 antibody may be used as a phenotypic marker for human CD28, expressed on all CD4+ T cells and CD8+ T cells, and is widely used as a reagent for activation of the CD28 receptor in vitro and in vivo (use format suitable for functional assays). This antibody is also reported to be cross-reactive with several non-human species, including Baboon, Chimpanzee, Cynomolgus, and Rhesus.

Human Anti-CD28 Antibody Product Attributes

Species: Human
Cross Reactivity: baboon, chimpanzee, cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD28 Antibody Clone: CD28.2
Clone CD28.2 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD28 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD28 / TP44 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD28 / TP44 could be seen in cells in the red pulp in spleen, germinal center cells in the tonsil, hematopoietic cells in the bone marrow and lymphoid tissue in appendix. We were unable to detect CD28 / TP44 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Griffin GK, Newton G, Tarrio ML, Bu D-X, Maganto-Garcia E, Azcutia V, Alcaide P, Grabie N, Luscinskas FW, Croce KJ, and Lichtman AH. 2012. J. Immunol. 188: 6287-6299. (in vitro activation)Cocchi F, DeVico AL, Lu W, Popovic M, Latiinovic O, Sajadi MM, Redfield RR, Lafferty MK, Galli M, Garzino-Demo A, and Gallo RC. 2012. Proc. Natl. Acad. Sci. 109: 5411-5416. (in vitro activation)Okoye AA, Rohankhedkar M, Abana C, Pattenn A, Reyes M, Pexton C, Lum R, Sylwester A, Planer SL, Legasse A, Park BS, Piatak M, Lifson JD, Axthelm MK and Picker LJ. 2012. J. Exp. Med. 209: 641-651. (Flow cytometry)Vanderford TH, Slichter C, Rogers KA, Lawson BO, Obaede R, Else J, Villinger F, Bosinger SE, and Silvestri G. 2012. Blood. 119: 5750-5757. (Flow cytometry - Sooty Mangabey)Ansari AA, Reimann KA, Mayne AE, Takahashi Y, Stephenson ST, Wang R, Wang X, Li J, Price AA, Little DM, Zaidi M, Lyles R, and Villinger F. 2011. J. Immunol. 186: 1044-1059. (Flow cytometry - Rhesus macaqueSoto PC, Stein LL, Hurtado-Ziola N, Hedrick SA, and Varki A. 2010. J. Immunol. 184: 4185-4195. (in vitro activation - Chimpanzee)Di Carlo E, D’Antuono T, Pompa P, Giuliani R, Rosini S, Stuppia L, Musiani P, and Sorrentino C. 2009. Clin. Cancer Res. 15: 2979-2987. (Immunohistochemistry - frozen tissue)Berg M and Zavazava N. 2008. J. Leukoc. Biol. 83: 852-863. (Immunoprecipitation)Fos C, Salles A, Lang V, Carrette F, Audebert S, Pastor S, Ghiotto M, Olive D, Bismuth G, and Nunes JA. 2008. J. Immunol. 181: 1969-1977. (Immunoprecipation, Flow cytometry)

Limitations and Warranty

enQuire Bio's Human Anti-CD28 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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