Human Anti-CD28 Antibody Product Attributes
Cross Reactivity: baboon, chimpanzee, cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD28 Antibody Clone: CD28.2
Clone CD28.2 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD28 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in CD28 / TP44 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD28 / TP44 could be seen in cells in the red pulp in spleen, germinal center cells in the tonsil, hematopoietic cells in the bone marrow and lymphoid tissue in appendix. We were unable to detect CD28 / TP44 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Griffin GK, Newton G, Tarrio ML, Bu D-X, Maganto-Garcia E, Azcutia V, Alcaide P, Grabie N, Luscinskas FW, Croce KJ, and Lichtman AH. 2012. J. Immunol. 188: 6287-6299. (in vitro activation)Cocchi F, DeVico AL, Lu W, Popovic M, Latiinovic O, Sajadi MM, Redfield RR, Lafferty MK, Galli M, Garzino-Demo A, and Gallo RC. 2012. Proc. Natl. Acad. Sci. 109: 5411-5416. (in vitro activation)Okoye AA, Rohankhedkar M, Abana C, Pattenn A, Reyes M, Pexton C, Lum R, Sylwester A, Planer SL, Legasse A, Park BS, Piatak M, Lifson JD, Axthelm MK and Picker LJ. 2012. J. Exp. Med. 209: 641-651. (Flow cytometry)Vanderford TH, Slichter C, Rogers KA, Lawson BO, Obaede R, Else J, Villinger F, Bosinger SE, and Silvestri G. 2012. Blood. 119: 5750-5757. (Flow cytometry - Sooty Mangabey)Ansari AA, Reimann KA, Mayne AE, Takahashi Y, Stephenson ST, Wang R, Wang X, Li J, Price AA, Little DM, Zaidi M, Lyles R, and Villinger F. 2011. J. Immunol. 186: 1044-1059. (Flow cytometry - Rhesus macaqueSoto PC, Stein LL, Hurtado-Ziola N, Hedrick SA, and Varki A. 2010. J. Immunol. 184: 4185-4195. (in vitro activation - Chimpanzee)Di Carlo E, DÂAntuono T, Pompa P, Giuliani R, Rosini S, Stuppia L, Musiani P, and Sorrentino C. 2009. Clin. Cancer Res. 15: 2979-2987. (Immunohistochemistry - frozen tissue)Berg M and Zavazava N. 2008. J. Leukoc. Biol. 83: 852-863. (Immunoprecipitation)Fos C, Salles A, Lang V, Carrette F, Audebert S, Pastor S, Ghiotto M, Olive D, Bismuth G, and Nunes JA. 2008. J. Immunol. 181: 1969-1977. (Immunoprecipation, Flow cytometry)
Limitations and Warranty
enQuire Bio's Human Anti-CD28 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.