Human Anti-CD31 / PECAM-1 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-CD31 / PECAM-1 Antibody Clone: 1A10
Clone 1A10 Host and Isotype: Mouse IgG1 kappa
Anti-Human CD31 / PECAM-1 Positive Control Sample: Jurkat cells. Tonsil or Angiosarcoma.
Cellular Localization of Antibody <1A10 Staining: Cell surface, cytoplasm of endothelial cells
Buffer and Stabilizer: Tissue culture supernatant with 0.05% Azide.
Antibody Concentration: Not applicable
Antibody Purification Method:Culture Supernatant
Immunogen: Prokaryotic recombinant fusion protein corresponding to a portion of the extracellular domain downstream of the signal sequence of the CD31 (PECAM-1)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
CD31 / PECAM-1 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD31 / PECAM-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in endothelial cells in the colon, cells in the glomeruli in kidney, smooth muscle cells in the smooth muscle and cells in the red pulp in spleen. More moderate antibody staining intensity was present in endothelial cells in the colon, cells in the glomeruli in kidney, smooth muscle cells in the smooth muscle and cells in the red pulp in spleen. Low, but measureable presence of CD31 / PECAM-1 could be seen inmacrophages in lung. We were unable to detect CD31 / PECAM-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Limitations and Warranty
enQuire Bio's CD31 / PECAM-1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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