Formalin-fixed, paraffin-embedded human Tonsil stained with CD31 Monoclonal Antibody (1A1)

Anti-CD31 / PECAM-1 Antibody Clone 1A10

$ 298.00
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SKU: 5175-MSM6
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
5175-MSM6-CS1 Size: 0.5 ml, Tag: Unconjugated
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SKU: 5175-MSM6-CS1
CD31 / PECAM-1 General Information
Alternate Names
Platelet endothelial cell adhesion molecule, PECAM-1, cluster of differentiation 31, CD31, PECAM
Molecular Weight
~100kDa (endothelium), ~130kDa (platelets)
Chromosomal Location
17q23.3
Curated Database and Bioinformatic Data
Gene SymbolPECAM1
Entrez Gene ID5175
Ensemble Gene IDENSG00000261371
RefSeq Protein Accession(s)XP_005276937, XP_016880229, NP_000433, XP_016880227, XP_016880228, XP_011523191, XP_005276938, XP_011523192, XP_005276939, XP_016880230, XP_005276940
RefSeq mRNA Accession(s)XM_011524890, XM_017024739, NM_000442, XM_005276881, XM_017024738, XM_005276880, XM_005276882, XM_011524889, XM_005276883, XM_017024740
RefSeq Genomic Accession(s)NG_047009, NC_000017, NC_018928
UniProt ID(s)P16284, A0A075B738
UniGene ID(s)P16284, A0A075B738
HGNC ID(s)8823
Cosmic ID(s)PECAM1
KEGG Gene ID(s)hsa:5175
PharmGKB ID(s)PA33167
General Description of CD31 / PECAM-1.
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system, is primarily used to identify, concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific, sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign,, malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis,, a high level of CD31 expression may imply a rapidly growing tumor, potentially a predictor of tumor recurrence.

Human Anti-CD31 / PECAM-1 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-CD31 / PECAM-1 Antibody Clone: 1A10
Clone 1A10 Host and Isotype: Mouse IgG1 kappa
Anti-Human CD31 / PECAM-1 Positive Control Sample: Jurkat cells. Tonsil or Angiosarcoma.
Cellular Localization of Antibody Cell surface, cytoplasm of endothelial cells
Buffer and Stabilizer: Tissue culture supernatant with 0.05% Azide.
Antibody Concentration: Not applicable
Antibody Purification Method:Culture Supernatant
Immunogen: Prokaryotic recombinant fusion protein corresponding to a portion of the extracellular domain downstream of the signal sequence of the CD31 (PECAM-1)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD31 / PECAM-1 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD31 / PECAM-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in endothelial cells in the colon, cells in the glomeruli in kidney, smooth muscle cells in the smooth muscle and cells in the red pulp in spleen. More moderate antibody staining intensity was present in endothelial cells in the colon, cells in the glomeruli in kidney, smooth muscle cells in the smooth muscle and cells in the red pulp in spleen. Low, but measureable presence of CD31 / PECAM-1 could be seen inmacrophages in lung. We were unable to detect CD31 / PECAM-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's CD31 / PECAM-1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD31 / PECAM-1 Antibody Clone 1A10