Human and Rat Anti-CD34 Antibody Product Attributes
Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 min at RT)
Anti-CD34 Antibody Clone: HPCA1/1171
Clone HPCA1/1171 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat CD34 Positive Control Sample: KG-1 cells, Tonsil, or Angiosarcoma
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human HPCA1 protein
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
CD34 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-CD34 antibody staining is expected to be primarily localized to the cell junctions, nucleus and plasma membrane.
Observed Antibody Staining Data By Tissue Type:
Variations in CD34 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the glomeruli in kidney and endothelial cells in the cerebral cortex and colon. More moderate antibody staining intensity was present in cells in the glomeruli in kidney and endothelial cells in the cerebral cortex and colon. Low, but measureable presence of CD34 could be seen in. We were unable to detect CD34 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's CD34 Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.