Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)
Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Monoclonal Antibody (HPCA1/1171)Flow Cytometry of KG-1 cells using CD34 Monoclonal Antibody (HPCA1/1171) (red) & isotype control (green).

Anti-CD34 Antibody Clone HPCA1/1171

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 947-MSM5
Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
947-MSM5-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 947-MSM5-P1
CD34 General Information
Alternate Names
Hematopoietic progenitor cell antigen CD34, CD34 antigen, CD34, Cluster of Differentiation 34
Molecular Weight
90-110kDa
Chromosomal Location
1q32.2
Curated Database and Bioinformatic Data
Gene SymbolCD34
Entrez Gene ID947
Ensemble Gene IDENSG00000174059
RefSeq Protein Accession(s)NP_001764, NP_001020280
RefSeq mRNA Accession(s)NM_001773, NM_001025109
RefSeq Genomic Accession(s)NC_018912, NC_000001
UniProt ID(s)P28906
UniGene ID(s)P28906
HGNC ID(s)1662
Cosmic ID(s)CD34
KEGG Gene ID(s)hsa:947
PharmGKB ID(s)PA26211
General Description of CD34.
This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor, gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma, Kaposi s sarcoma, but shows low specificity.

Human and Rat Anti-CD34 Antibody Product Attributes

Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-CD34 Antibody Clone: HPCA1/1171
Clone HPCA1/1171 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat CD34 Positive Control Sample: KG-1 cells, Tonsil, or Angiosarcoma
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human HPCA1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD34 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CD34 antibody staining is expected to be primarily localized to the cell junctions, nucleus and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in CD34 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the glomeruli in kidney and endothelial cells in the cerebral cortex and colon. More moderate antibody staining intensity was present in cells in the glomeruli in kidney and endothelial cells in the cerebral cortex and colon. Low, but measureable presence of CD34 could be seen in. We were unable to detect CD34 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's CD34 Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD34 Antibody Clone HPCA1/1171

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