Mouse Anti-CD357 / GITR / TNFRSF18 Antibody Product Attributes
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD357 / GITR / TNFRSF18 Antibody Clone: DTA-1
Clone DTA-1 Host and Isotype: Rat IgG2b
Buffer and Stabilizer: 10mM Tris, 150 mM NaCl, 0.05% BSA, 0.05% NaN3, pH8
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD357 / GITR / TNFRSF18 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in CD357 / GITR / TNFRSF18 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD357 / GITR / TNFRSF18 could be seen inhematopoietic cells in the bone marrow, glandular cells in the breast, colon and gallbladder, pneumocytes in lung, germinal center cells in the lymph node, respiratory epithelial cells in the nasopharynx, exocrine glandular cells in the pancreas, glandular cells in the parathyroid gland and rectum, keratinocytes in skin, cells in the red pulp in spleen, glandular cells in the stomach and thyroid gland and squamous epithelial cells in the tonsil. We were unable to detect CD357 / GITR / TNFRSF18 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of CD357 / GITR / TNFRSF18 expression as measured by anti-CD357 / GITR / TNFRSF18 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Lee L-F, Logronio K, Tu GH, Zhai W, Ni I, Mei L, Dilley J, Yu J, et al. 2012. Proc. Natl. Acad. Sci. 10.1073. (Flow cytometry).Joetham A, Ohnishi H, Okamoto M, Takeda K, Schedel M, Domenico J, Dakhama A, and Gelfand EW. 2012. J. Biol. Chem. 287: 17100-17108. (in vitro activation)Van der Werf N, Redpath SA, Phythian-Adams AT, Azuma M, Allen JE, Maizels RM, Macdonald AS, and Taylor MD. 2011. J. Immunol. 187: 1411-1420. (in vivo activation)Molloy MJ, Zhang W, and Usherwood EJ. 2011. J. Immunol. 186: 6218-6226. (in vivo activation) llYokoyama T, Matsuda S, Takae Y, Wada N, Nishikawa T, Amagai M, and Koyasu S. 2011. Int. Immunol. 23: 365-373. (Treg depletion - magnetic beads)
Limitations and Warranty
enQuire Bio's Mouse Anti-CD357 / GITR Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.