C57Bl/6 splenocytes were stained with FITC Mouse Anti-CD4 , PE Mouse Anti-CD25  and 0.06 ug APC Mouse Anti-CD357 (GITR) (QAB72). Cells in the CD4+ lymphocyte gate are shown.
C57Bl/6 splenocytes were stained with FITC Mouse Anti-CD4 , PE Mouse Anti-CD25  and 0.06 ug APC Mouse Anti-CD357 (GITR) (QAB72). Cells in the CD4+ lymphocyte gate are shown.C57Bl/6 splenocytes were stained with FITC Mouse Anti-CD4 , APC Mouse Anti-CD25  and 0.004 ug PE Mouse Anti-CD357 . Cells in the CD4+ lymphocyte gate are shown.

Anti-CD357 / GITR Antibody [DTA-1]

$ 89.00$ 259.00
Please Select A Product To View The SKU.

SKU: QAB72
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;APC ;PE
Isotype: Rat IgG2b
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB72-F-25ug
CD357 / GITR / TNFRSF18 General Information
Alternate Names
GITR, TNFRSF18, AITR, GITR-D, GITR, CD357
Curated Database and Bioinformatic Data
Gene SymbolTnfrsf18
Entrez Gene ID21936
Ensemble Gene IDENSMUSG00000041954
RefSeq Protein Accession(s)XP_017175602, NP_068820, NP_033426
RefSeq mRNA Accession(s)NM_021985, NM_009400, XM_017320113
RefSeq Genomic Accession(s)NC_000070
UniProt ID(s)Q540M6, O35714
UniGene ID(s)Q540M6, O35714
Cosmic ID(s)Tnfrsf18
KEGG Gene ID(s)mmu:21936
General Description of CD357 / GITR / TNFRSF18.
The DTA-1 antibody reacts with mouse CD357, also known as GITR or AITR (in humans), a 66-70 kDa member of the Tumor Necrosis Factor superfamily (TNFRSF18). GITR is primarily found on T cells, and its function may vary depending on the T cell type where it is expressed. GITR is upregulated on activated T cells where it provides co-stimulation, yet GITR may promote the inhibition of CD4+ CD25+ Treg cells, where it is expressed at high levels. GITR ligand (GITRL) is found on B cells, macrophages, dendritic and endothelial cells, and is implicated in regulating both innate and adaptive immune responses.The DTA-1 antibody may be used for analysis of GITR expression on T cells, and is also commonly used in vitro as an agonistic antibody to induce GITR signaling in various assays (use format appropriate for functional assays).

Mouse Anti-CD357 / GITR / TNFRSF18 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD357 / GITR / TNFRSF18 Antibody Clone: DTA-1
Clone DTA-1 Host and Isotype: Rat IgG2b
Buffer and Stabilizer: 10mM Tris, 150 mM NaCl, 0.05% BSA, 0.05% NaN3, pH8
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD357 / GITR / TNFRSF18 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD357 / GITR / TNFRSF18 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD357 / GITR / TNFRSF18 could be seen inhematopoietic cells in the bone marrow, glandular cells in the breast, colon and gallbladder, pneumocytes in lung, germinal center cells in the lymph node, respiratory epithelial cells in the nasopharynx, exocrine glandular cells in the pancreas, glandular cells in the parathyroid gland and rectum, keratinocytes in skin, cells in the red pulp in spleen, glandular cells in the stomach and thyroid gland and squamous epithelial cells in the tonsil. We were unable to detect CD357 / GITR / TNFRSF18 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD357 / GITR / TNFRSF18 expression as measured by anti-CD357 / GITR / TNFRSF18 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + - + + - - + - - ++ - - + - - - - - - -
TNFRSF18 Variability ++ ++ ++ ++ ++ + + ++ ++ ++ ++ ++ ++ + ++ + ++ + ++ ++

Selected References

Lee L-F, Logronio K, Tu GH, Zhai W, Ni I, Mei L, Dilley J, Yu J, et al. 2012. Proc. Natl. Acad. Sci. 10.1073. (Flow cytometry).Joetham A, Ohnishi H, Okamoto M, Takeda K, Schedel M, Domenico J, Dakhama A, and Gelfand EW. 2012. J. Biol. Chem. 287: 17100-17108. (in vitro activation)Van der Werf N, Redpath SA, Phythian-Adams AT, Azuma M, Allen JE, Maizels RM, Macdonald AS, and Taylor MD. 2011. J. Immunol. 187: 1411-1420. (in vivo activation)Molloy MJ, Zhang W, and Usherwood EJ. 2011. J. Immunol. 186: 6218-6226. (in vivo activation) llYokoyama T, Matsuda S, Takae Y, Wada N, Nishikawa T, Amagai M, and Koyasu S. 2011. Int. Immunol. 23: 365-373. (Treg depletion - magnetic beads)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD357 / GITR Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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