C57Bl/6 splenocytes were stained with 0.06 ug APC Mouse Anti-CD4 (QAB8) (solid line) or 0.06 ug APC Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Mouse Anti-CD4 Antibody [GK1.5]

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Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: Biotin ;Unconjugated ;APC ;APC-Cy7 ;FITC ;PerCP-Cy5.5 ;PE ;PE-Cy5 ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB8-B-25ug
CD4 General Information
Alternate Names
CD4, Leu-3, CD4mut
Curated Database and Bioinformatic Data
Gene SymbolCd4
Entrez Gene ID12504
Ensemble Gene IDENSMUSG00000023274
RefSeq Protein Accession(s)XP_006505524, XP_006505525, NP_038516
RefSeq mRNA Accession(s)NM_013488, XM_006505462, XM_006505461
RefSeq Genomic Accession(s)NC_000072
UniProt ID(s)Q3TSV7, P06332
UniGene ID(s)Q3TSV7, P06332
Cosmic ID(s)Cd4
KEGG Gene ID(s)mmu:12504
General Description of CD4.
The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Mouse Anti-CD4 clone RM4-5, will compete for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Mouse Anti-CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4.

Mouse Anti-CD4 Antibody Product Attributes

Species: Mouse
Cross Reactivity: syrian hamster
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD4 Antibody Clone: GK1.5
Clone GK1.5 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD4 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD4 antibody reveals CD4 expression is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in CD4 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD4 could be seen in cells in the white pulp in spleen and germinal center cells in the tonsil. We were unable to detect CD4 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Taniguchi RT, DeVoss JJ, Moon JJ, Sidney J, Sette A, Jenkins MK, and Anderson MS. 2012. Proc. Natl. Acad. Sci. 109: 7847-7852. (in vivo depletion)Wolkers MC, Gerlach, C, Arens R, Janssen EM, Fitzgerald P, Schumacher TN, Medema JP, Green DR, and Schoenberger SP. 2012. Blood. 119:798-804. (in vivo depletion)Lee L-F, Logronio K, Tu GH, Zhai W, Ni I, Mei L, Dilley J, Yu J, et al. 2012. Proc. Natl. Acad. Sci. 10.1073. (Flow cytometry).Thornton EE, Looney MR, Bose O, Sen D, Sheppard D, Locksley R, Huang X, and Krummel MF. 2012. J. Exp. Med. 10:1084. (Immunohistochemistry - OCT embedded frozen tissue)Stephen TL, Wilson BS, and Laufer TM. 2012. Proc. Natl. Acad. Sci. 109: 7415-7420. (Immunoprecipitation)Hammerbeck CD and Hooper JW. 2011. J. Virol. 85(19) 9929-9944. (Flow cytometry - Syrian hamster).Diamond MS, Kinder M, Matsushita H, Mashayekhi M, Dunn GP, Archambault JM, Lee H, Arthur CD, White JM, Kalinke U, Murphy KM, and Schreiber RD. 2011. J. Exp. Med. 208: 1989-2003. (in vitro blocking)Kao H, Lin J, Littman DR, Shaw AS, and Allen PM. 2008. J. Immunol. 181: 8248-8257. (Immunoprecipitation)Felix NJ, Donermeyer DL, Horvath S, Walters JJ, Gross M, suri A, and Allen PM. 2007. Nat. Immunol. 8:388-397. (in vitro blocking)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD4 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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