Human PBMCs were stained with 5 uL  (solid line) or 0.5 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Human Anti-CD4 Antibody [RPA-T4]

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SKU: QAB12
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;APC ;APC-Cy7 ;PerCP-Cy5.5 ;PE ;PE-Cy7 ;V450
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB12-F-25Tests
CD4 General Information
Alternate Names
CD4, Leu-3, CD4mut
Curated Database and Bioinformatic Data
Gene SymbolCD4
Entrez Gene ID920
Ensemble Gene IDENSG00000010610
RefSeq Protein Accession(s)NP_000607, NP_001181946, NP_001181944, XP_016875717, NP_001181943, NP_001181945
RefSeq mRNA Accession(s)NM_001195017, XM_017020228 NM_001195015, NR_036545, NM_001195016, NM_000616, NM_001195014
RefSeq Genomic Accession(s)NC_018923, NG_027688, NC_000012,
UniProt ID(s)B0AZV7, P01730, B4DT49
UniGene ID(s)B0AZV7, P01730, B4DT49
HGNC ID(s)1678
Cosmic ID(s)CD4
KEGG Gene ID(s)hsa:920
PharmGKB ID(s)PA26220
General Description of CD4.
The RPA-T4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RPA-T4 antibody is widely used as a phenotypic marker for human CD4 expression, and is cross-reactive with Chimpanzee CD4. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Human Anti-CD4 antibody clone OKT4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065).

Human Anti-CD4 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD4 Antibody Clone: RPA-T4
Clone RPA-T4 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD4 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD4 antibody reveals CD4 expression is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in CD4 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD4 could be seen in cells in the white pulp in spleen and germinal center cells in the tonsil. We were unable to detect CD4 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Toma J, Weinheimer SP, Stawiski E, Whitcomb JM, Lewis ST, Petropoulos CJ, and Huang W. 2011. J. Virol. 85: 3872-3880. (Blocking: HIV-1 interaction)Porter KA, Kelley LN, Nekorchuk MD, Jones JH, Hahn AB, de Noronha CMC, Harton JA, and Duus KM. 2010. J. Immunol. 185:6480-6488. (Blocking: HIV-1 interaction)Hsieh S-C, Tsai W-Y, and Wang W-K. 2010. J. Virol. 84(9): 4782-4797. (Immunoprecipitation - transfected cells)Chen X, Wang X, Besra GS, and Gumperz JE. 2007. J. Leukoc. Biol. 82:1455-1465. (in vitro activation)Thedrez A, de Lalla C, Allain S, Zaccagnino L, et al. 2007. Blood. 110:251-258 (in vitro blocking)Mack CL, Tucker RM, Sokol RJ, Darrer FM, Kotzin BL, Whitington PF and Miller SD. 2004. Pediatr. Res. 56(1):79-87. (Immunohistochemistry - frozen tissue)Deng MC, Bell S, Huie P, Pinto F, Hunt SA, Stinson EB, Sibley R, Hall BM, and Valantine HA. 1995. Circulation. 91: 1647-1654. (Immunohistochemistry - OCT embedded frozen tissue)

Limitations and Warranty

enQuire Bio's Human Anti-CD4 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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