Human PBMCs were stained with 5 uL  (solid line) or 0.06 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.
Human PBMCs were stained with 5 uL  (solid line) or 0.06 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  (solid line) or 0.06 ug PerCP Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  .

Anti-CD4 Antibody [SK3]

$ 178.00$ 2,558.00
Please Select Product Options Below To View The Catalog Number.

Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;APC ;PerCP ;PerCP-Cy5.5 ;PE-Cy7
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
QAB10-F-25Tests Size: 25 tests, Tag: FITC $ 159.00
QAB10-APC-100Tests Size: 100 tests, Tag: APC $ 419.00
QAB10-APC-25Tests Size: 25 tests, Tag: APC $ 189.00
QAB10-APC-500Tests Size: 500 tests, Tag: APC $ 1,889.00
QAB10-F-100Tests Size: 100 tests, Tag: FITC $ 299.00
QAB10-F-500Tests Size: 500 tests, Tag: FITC $ 1,329.00
QAB10-PCP-100Tests Size: 100 tests, Tag: PerCP $ 449.00
QAB10-PCP-25Tests Size: 25 tests, Tag: PerCP $ 169.00
QAB10-PCP-500Tests Size: 500 tests, Tag: PerCP $ 1,979.00
QAB10-PCP55-100Tests Size: 100 tests, Tag: PerCP-Cy5.5 $ 519.00
QAB10-PCP55-25Tests Size: 25 tests, Tag: PerCP-Cy5.5 $ 209.00
QAB10-PCP55-500Tests Size: 500 tests, Tag: PerCP-Cy5.5 $ 2,279.00
QAB10-PE7-100Tests Size: 100 tests, Tag: PE-Cy7 $ 379.00
QAB10-PE7-25Tests Size: 25 tests, Tag: PE-Cy7 $ 189.00
QAB10-PE7-500Tests Size: 500 tests, Tag: PE-Cy7 $ 1,699.00
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:


Bioactivity (test results eg. IU/ml):

SKU: QAB10-F-25Tests
CD4 General Information
Alternate Names
CD4, Leu-3, CD4mut
Curated Database and Bioinformatic Data
Gene SymbolCD4
Entrez Gene ID920
Ensemble Gene IDENSG00000010610
RefSeq Protein Accession(s)NP_000607, NP_001181946, NP_001181944, XP_016875717, NP_001181943, NP_001181945
RefSeq mRNA Accession(s)NM_001195017, XM_017020228 NM_001195015, NR_036545, NM_001195016, NM_000616, NM_001195014
RefSeq Genomic Accession(s)NC_018923, NG_027688, NC_000012,
UniProt ID(s)B0AZV7, P01730, B4DT49
UniGene ID(s)B0AZV7, P01730, B4DT49
HGNC ID(s)1678
Cosmic ID(s)CD4
KEGG Gene ID(s)hsa:920
PharmGKB ID(s)PA26220
General Description of CD4.
The SK3 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells. The SK3 antibody is widely used as a phenotypic marker for human CD4 expression, and has been reported to be cross-reactive with Rhesus and Cynomolgus CD4. This antibody does not block binding of alternative clone RPA-T4, suggesting that they recognize different epitopes.

Human Anti-CD4 Antibody Product Attributes

Species: Human
Cross Reactivity: cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD4 Antibody Clone: SK3
Clone SK3 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD4 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD4 antibody reveals CD4 expression is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in CD4 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD4 could be seen in cells in the white pulp in spleen and germinal center cells in the tonsil. We were unable to detect CD4 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Evans RL, Wall DW, Platsoucas CD, Siegal FP, Fikrig SM, Testa CM and Good RA. 1981. Proc Natl Acad Sci U S A. 78(1): 544-548.Sattentau QJ, Dalgleish AG, Weiss RA and Beverley PC. 1986. Science. 234(4780): 1120-1123. Bernard A, Boumsell L and Hill C. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF, ed. Leucocyte Typing. New York, NY: Springer-Verlag; 1984: 9-108. (Flow Cytometry) Heninger AK, Theil A, Wilhelm C, Petzold C, Huebel N, Kretschmer K, Bonifacio E and Monti P. 2012. J Immunol. 189(12): 5649-5648. (Flow Cytometry) Yoshino N, Ami Y, Terao K, Tashiro F and Honda M. 2000. Exp. Anim. 49(2): 97-110. (Flow Cytometry - Cynomolgus) Lafont BAP, Gloeckler L, D'Hautcourt JL, Gut JP and Aubertin AM. 2000. Cytometry 41: 193-202. (Flow Cytometry - Rhesus)

Limitations and Warranty

enQuire Bio's Human Anti-CD4 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-CD4 Antibody [SK3]”

Your email address will not be published.

Share a Protocol (View Reviewed Protocols Below)

Protocol Title




  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Chat: See lower right corner. | | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Anti-CD4 Antibody [SK3]