Human Anti-CD4 Antibody Product Attributes
Cross Reactivity: cynomolgus monkey, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD4 Antibody Clone: SK3
Clone SK3 Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD4 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Staining with anti-CD4 antibody reveals CD4 expression is expected to be primarily localized to the plasma membrane.
Observed Antibody Staining Data By Tissue Type:
Variations in CD4 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD4 could be seen in cells in the white pulp in spleen and germinal center cells in the tonsil. We were unable to detect CD4 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Evans RL, Wall DW, Platsoucas CD, Siegal FP, Fikrig SM, Testa CM and Good RA. 1981. Proc Natl Acad Sci U S A. 78(1): 544-548.Sattentau QJ, Dalgleish AG, Weiss RA and Beverley PC. 1986. Science. 234(4780): 1120-1123. Bernard A, Boumsell L and Hill C. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF, ed. Leucocyte Typing. New York, NY: Springer-Verlag; 1984: 9-108. (Flow Cytometry) Heninger AK, Theil A, Wilhelm C, Petzold C, Huebel N, Kretschmer K, Bonifacio E and Monti P. 2012. J Immunol. 189(12): 5649-5648. (Flow Cytometry) Yoshino N, Ami Y, Terao K, Tashiro F and Honda M. 2000. Exp. Anim. 49(2): 97-110. (Flow Cytometry - Cynomolgus) Lafont BAP, Gloeckler L, D'Hautcourt JL, Gut JP and Aubertin AM. 2000. Cytometry 41: 193-202. (Flow Cytometry - Rhesus)
Limitations and Warranty
enQuire Bio's Human Anti-CD4 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.