Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CD44 Monoclonal Antibody (DF1485)
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CD44 Monoclonal Antibody (DF1485)

Anti-CD44 / HCAM Antibody Clone DF1485

$ 368.00
Please Select Product Options Below To View The Catalog Number.

SKU: 960-MSM3
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
960-MSM3-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
Flat Rate Shipping Anywhere in the US: $45
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SKU: 960-MSM3-P1
CD44 / HCAM General Information
Alternate Names
CD44, Cluster of Differentiation 44
Molecular Weight
80-95kDa
Chromosomal Location
11p13
Curated Database and Bioinformatic Data
Gene SymbolCD44
Entrez Gene ID960
Ensemble Gene IDENSG00000026508
RefSeq Protein Accession(s)XP_005253290, XP_011518789, XP_011518790, XP_005253288, XP_011518784, XP_011518786, NP_001001391, XP_005253297, XP_006718451, XP_011518787, XP_011518791, NP_000601, NP_001001389, NP_001189486, XP_005253289, XP_011518788, NP_001001392, NP_001189484, XP_005253292, XP_005253295, XP_005253296, XP_011518785, NP_001001390, XP_005253291, XP_006718453, XP_016874072, XP_016874074, NP_001189485, XP_016874073
RefSeq mRNA Accession(s)NM_001202556, XM_005253235, XM_006718388, NM_001001389, NM_001001391, XM_011520489, XM_011520485, XM_011520487, XM_011520488, XM_017018584, XM_017018585, NM_001202555, XM_005253231, XM_005253232, XM_005253234, XM_006718390, XM_011520483, NM_001001392, NM_001202557, XM_005253238, XM_005253239, XM_005253240, NM_001001390, XM_005253233, XM_011520482, XM_011520484, XM_011520486, XM_017018583, NM_000610
RefSeq Genomic Accession(s)NG_008937, NC_000011, NC_018922
UniProt ID(s)P16070
UniGene ID(s)P16070
HGNC ID(s)1681
Cosmic ID(s)CD44
KEGG Gene ID(s)hsa:960
PharmGKB ID(s)PA26221
General Description of CD44 / HCAM.
Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes,, granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin, chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion, stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Human and Mouse Anti-CD44 / HCAM Antibody Product Attributes

Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD44 / HCAM Antibody Clone: DF1485
Clone DF1485 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Mouse CD44 / HCAM Positive Control Sample: HeLa cells or paracortex in tonsil or lymph node.
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Purified CD44 antigen (PGp-1) from lymphocyte membrane
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD44 / HCAM Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CD44 antibody staining is expected to be primarily localized to the golgi apparatus and plasma membrane. There is variability in either the signal strength or the localization of signal in golgi apparatus from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in CD44 / HCAM antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in epidermal cells in the skin, glandular cells in the breast, cervix, uterine, prostate and salivary gland, hematopoietic cells in the bone marrow, keratinocytes in skin, Langerhans in skin, melanocytes in skin, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in epidermal cells in the skin, glandular cells in the breast, cervix, uterine, prostate and salivary gland, hematopoietic cells in the bone marrow, keratinocytes in skin, Langerhans in skin, melanocytes in skin, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina and urothelial cells in the urinary bladder. Low, but measureable presence of CD44 / HCAM could be seen inadipocytes in mesenchymal tissue, cells in the glomeruli in kidney, cells in the tubules in kidney, decidual cells in the placenta, endothelial cells in the cerebral cortex and colon, follicle cells in the ovary, glandular cells in the fallopian tube, rectum and seminal vesicle, Leydig cells in the testis, neuronal cells in the cerebral cortex, neuropil in cerebral cortex, pneumocytes in lung and trophoblastic cells in the placenta. We were unable to detect CD44 / HCAM in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD44 / HCAM expression as measured by anti-CD44 / HCAM antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ - +++ ++ ++ +++ +++ + + +++ +++ + ++ ++ - ++ - - ++ -
CD44 Variability ++ + + ++ ++ ++ + ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++

Limitations and Warranty

enQuire Bio's CD44 / HCAM Std. Anti-Human, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD44 / HCAM Antibody Clone DF1485

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