C57Bl/6 splenocytes were stained with 0.125 ug Anti-Hu/Mo CD44 APC (QAB39) (solid line) or 0.125 ug Rat IgG2b APC isotype control (dashed line). Flow Cytometry Data from 10,000 events.
C57Bl/6 splenocytes were stained with 0.125 ug Anti-Hu/Mo CD44 APC (QAB39) (solid line) or 0.125 ug Rat IgG2b APC isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.25 ug APC-Cy7 Anti-Hu/Mo CD44 .C57Bl/6 splenocytes were stained with 0.5 ug FITC Anti-Hu/Mo CD44 (QAB39) (solid line) or 0.5 ug FITC Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.25 ug PerCP-Cy5.5 Anti-Hu/Mo CD44 .C57Bl/6 splenocytes were stained with 0.125 ug Anti-Hu/Mo CD44 PE (QAB39) (solid line) or 0.125 ug Rat IgG2b PE isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0125 ug PE-Cy5 Anti-Hu/Mo CD44 .C57Bl/6 splenocytes were stained with 0.125 ug PE-Cy7 Anti-Hu/Mo CD44 .C57Bl/6 splenocytes were stained with 0.5 ug Qfluor™ 710 Anti-Hu/Mo CD44 (QAB39) (solid line) or 0.5 ug Qfluor™ 710 Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.5 ug V450 Anti-Hu/Mo CD44  (solid line) or 0.5 ug V450 Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Anti-Human/Mouse CD44 Antibody [IM7]

$ 48.00$ 208.00
Please Select Product Options Below To View The Catalog Number.

SKU: QAB39
Species: Human and Mouse
Tested Applications: Flow Cytometry
Available Conjugates: Biotin ;Unconjugated ;APC ;APC-Cy7 ;FITC ;PerCP-Cy5.5 ;PE ;PE-Cy5 ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
QAB39-B-25ug Size: 25 ug, Tag: Biotin $ 48.00
QAB39-100ug Size: 100 ug, Tag: Unconjugated $ 88.00
QAB39-APC-100ug Size: 100 ug, Tag: APC $ 158.00
QAB39-APC-25ug Size: 25 ug, Tag: APC $ 58.00
QAB39-APC7-100ug Size: 100 ug, Tag: APC-Cy7 $ 178.00
QAB39-APC7-25ug Size: 25 ug, Tag: APC-Cy7 $ 78.00
QAB39-B-100ug Size: 100 ug, Tag: Biotin $ 108.00
QAB39-F-100ug Size: 100 ug, Tag: FITC $ 88.00
QAB39-F-25ug Size: 25 ug, Tag: FITC $ 48.00
QAB39-F-500ug Size: 500 ug, Tag: FITC $ 148.00
QAB39-PCP55-100ug Size: 100 ug, Tag: PerCP-Cy5.5 $ 178.00
QAB39-PCP55-25ug Size: 25 ug, Tag: PerCP-Cy5.5 $ 88.00
QAB39-PE-100ug Size: 100 ug, Tag: PE $ 98.00
QAB39-PE-25ug Size: 25 ug, Tag: PE $ 48.00
QAB39-PE5-100ug Size: 100 ug, Tag: PE-Cy5 $ 138.00
QAB39-PE5-25ug Size: 25 ug, Tag: PE-Cy5 $ 68.00
QAB39-PE7-100ug Size: 100 ug, Tag: PE-Cy7 $ 168.00
QAB39-PE7-25ug Size: 25 ug, Tag: PE-Cy7 $ 78.00
QAB39-QF710-100ug Size: 100 ug, Tag: Qfluor™ 710 $ 168.00
QAB39-QF710-25ug Size: 25 ug, Tag: Qfluor™ 710 $ 78.00
QAB39-V450-100ug Size: 100 ug, Tag: V450 $ 208.00
QAB39-V450-25ug Size: 25 ug, Tag: V450 $ 98.00
QAB39-QF630-100ug Size: 100 tests, Tag: Qfluor™ 630 $ 168.00
Shipping Information
In Stock.
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Datasheets and Documentation
Product Datasheet
SKU: QAB39-B-25ug
CD44 / HCAM General Information
Alternate Names
CD44, HCAM, CDW44, HUTCH-I, IN, LHR, CSPG8, MC56, MDU2, MDU3, ECMR-III, MIC4, Pgp1, HCELL
Curated Database and Bioinformatic Data
Gene SymbolCd44
Entrez Gene ID12505
Ensemble Gene IDENSMUSG00000005087
RefSeq Protein Accession(s)XP_006498711, NP_001171256, NP_001171257, XP_006498709, NP_033981, XP_006498708, XP_006498712, NP_001034240, NP_001171258, XP_006498710, NP_001034239
RefSeq mRNA Accession(s)XM_006498649, NM_009851, XM_006498647, XM_006498645, XM_006498646, NM_001039150, NM_001177787, NM_001039151, XM_006498648, NM_001177785, NM_001177786
RefSeq Genomic Accession(s)NC_000068
UniProt ID(s)P15379, A2APM1, Q3U8S1, A2APM2, Q80X37, A2APM4, A2APM3
UniGene ID(s)P15379, A2APM1, Q3U8S1, A2APM2, Q80X37, A2APM4, A2APM3
Cosmic ID(s)Cd44
KEGG Gene ID(s)mmu:12505
General Description of CD44 / HCAM.
The IM7 antibody recognizes CD44, a ubiquitously expressed cell surface receptor which is important for extracellular matrix organization, cell-cell and cell-matrix adhesion and migration. CD44 may be expressed in a number of different isoforms (splice variants) from the most typical or standard form, known as CD44s, to variants designated CD44v, e.g. CD44v1 or CD44v6. These receptors interact with several ligands, but most often associate with an extracellular matrix component hyaluronate, through which it mediates adhesion.The IM7 antibody may be used for detection of all isoforms of CD44, as it recognizes constant epitopes near the extracellular proximal domain. (Xu et al, 2002, J. Leukoc. Biol. 72:1133-1141). It has been reported to be cross-reactive with many non-human species including Baboon, Chimpanzee, Cynomolgus, Rhesus, Horse, Cow, Pig, Canine and Cat CD44.

Human and Mouse Anti-CD44 / HCAM Antibody Product Attributes

Species: Human and Mouse
Cross Reactivity: baboon, Canine, chimpanzee, cynomolgus monkey, horse, cat, rhesus macaque, pig
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD44 / HCAM Antibody Clone: IM7
Clone IM7 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD44 / HCAM Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Staining with anti-CD44 / HCAM antibody reveals CD44 / HCAM expression is expected to be primarily localized to the golgi apparatus and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in CD44 / HCAM antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in epidermal cells in the skin, glandular cells in the breast, cervix, uterine, prostate and salivary gland, hematopoietic cells in the bone marrow, keratinocytes in skin, Langerhans in skin, melanocytes in skin, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in epidermal cells in the skin, glandular cells in the breast, cervix, uterine, prostate and salivary gland, hematopoietic cells in the bone marrow, keratinocytes in skin, Langerhans in skin, melanocytes in skin, respiratory epithelial cells in the bronchus and nasopharynx, squamous epithelial cells in the cervix, uterine, esophagus, oral mucosa, tonsil and vagina and urothelial cells in the urinary bladder. Low, but measureable presence of CD44 / HCAM could be seen inadipocytes in mesenchymal tissue, cells in the glomeruli in kidney, cells in the tubules in kidney, decidual cells in the placenta, endothelial cells in the cerebral cortex and colon, follicle cells in the ovary, glandular cells in the fallopian tube, rectum and seminal vesicle, Leydig cells in the testis, neuronal cells in the cerebral cortex, neuropil in cerebral cortex, pneumocytes in lung and trophoblastic cells in the placenta. We were unable to detect CD44 / HCAM in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD44 / HCAM expression as measured by anti-CD44 / HCAM antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ - +++ ++ ++ +++ +++ + + +++ +++ + ++ ++ - ++ - - ++ -
CD44 Variability ++ + + ++ ++ ++ + ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++

Selected References

Chandler HL, Haeussler DJ, Gemensky-Metzler AJ, Wilkie DA, and Lutz EA. 2012. Invest. Ophthalmol. Vis. Sci. 53:1835-1845. (in vitro blocking, canine)Lee L-F, Logronio K, Tu GH, Zhai W, Ni I, Mei L, Dilley J, Yu J, et al. 2012. Proc. Natl. Acad. Sci. 10.1073. (Flow cytometry).Ruffell B, Poon GFT, Lee SSM, Brown KL, Tjew S-L, Cooper J, and Johnson P. 2011. J. Biol. Chem. 286:19179-19190. (Immunoprecipitation)Miyake Y, Matsumoto H, Yokoo M, Miyazawa K et al. 2006. Biol. Reprod. 74: 501-510. (Immunohistochemistry - frozen tissue, swine)Veir JK, Lappin MR, and Dow SW. 2006. Journal of Feline Medicine and Surgery. 8:400-411. (Flow cytometry - feline)Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, and Frank MH. 2005. Cancer Res. 65:4320-4333. (Immunohistochemistry - frozen tissue)Fischer A, Schumacher N, Maier M, Sendtner M, and Gessler M. 2004. Genes & Dev. 18:901-911. (Immunohistochemistry - paraffin embedded tissue)Xu H, Manivannan A, Liversidge J, Sharp PF, Forrester JV, and Crane IJ. 2002. J. Leukoc. Biol. 72:1133-1141. (in vivo functional assays, induction of apoptosis)Si-Tahar M, Sitaraman S, Shibahara T, and Madara JL. 2001. Am. J. Physiol. Cell Physiol. 280:C423-C432. (in vitro functional assays, Western Blot)

Limitations and Warranty

enQuire Bio's Anti-Human/Mouse CD44 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Human/Mouse CD44 Antibody [IM7]

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