C57Bl/6 splenocytes were stained with 0.125 ug APC Mouse Anti-CD45 (QAB40) (solid line) or 0.125 ug APC Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.
C57Bl/6 splenocytes were stained with 0.125 ug APC Mouse Anti-CD45 (QAB40) (solid line) or 0.125 ug APC Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.2 ug FITC Mouse Anti-CD45  (solid line) or 0.2 ug FITC Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.06 ug PE Mouse Anti-CD45 (QAB40) (solid line) or 0.06 ug PE Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.125 ug PE-Cy7 Mouse Anti-CD45 .C57Bl/6 splenocytes were stained with 0.25 ug Qfluor™ 710 Mouse Anti-CD45 (QAB40) (solid line) or 0.25 ug Qfluor™ 710 Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.25 ug V450 Mouse Anti-CD45  (solid line) or 0.25 ug V450 Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.125 ug APC Mouse Anti-CD45 (QAB40) (solid line) or 0.125 ug APC Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.2 ug FITC Mouse Anti-CD45  (solid line) or 0.2 ug FITC Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.06 ug PE Mouse Anti-CD45 (QAB40) (solid line) or 0.06 ug PE Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.125 ug PE-Cy7 Mouse Anti-CD45 .C57Bl/6 splenocytes were stained with 0.25 ug Qfluor™ 710 Mouse Anti-CD45 (QAB40) (solid line) or 0.25 ug Qfluor™ 710 Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.C57Bl/6 splenocytes were stained with 0.25 ug V450 Mouse Anti-CD45  (solid line) or 0.25 ug V450 Rat IgG2b isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Anti-CD45 Antibody [30-F11]

$ 49.00$ 179.00
Please Select A Product To View The SKU.

SKU: QAB40
Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: Biotin ;Unconjugated ;APC ;FITC ;PE ;PE-Cy7 ;Qfluor 710 ;V450
Isotype: Rat IgG2b kappa
Mass Spec Validated?: Not MS Validated

Size
Tag Clear selection
Shipping Information
In Stock: 1-2 Day Lead Time.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: QAB40-B-25ug
CD45 General Information
Alternate Names
CD45, PTPRC, Lyt-4, T2, loc, CD45R, Ly-5, B22, Cd45, L-CA, LCA
Curated Database and Bioinformatic Data
Gene SymbolPtprc
Entrez Gene ID19264
Ensemble Gene IDENSMUSG00000026395
RefSeq Protein Accession(s)NP_035340, XP_006529324, XP_006529321, XP_006529323, NP_001104786, XP_006529320, XP_006529322, XP_006529325, NP_001255215
RefSeq mRNA Accession(s)XM_006529259, XM_006529262, NM_011210, XM_006529260, NM_001111316, XM_006529257, XM_006529258, XM_006529261, NM_001268286
RefSeq Genomic Accession(s)NC_000067
UniProt ID(s)S4R1M0, S4R1S4, S4R2V1, Q8C6Q7
UniGene ID(s)S4R1M0, S4R1S4, S4R2V1, Q8C6Q7
Cosmic ID(s)Ptprc
KEGG Gene ID(s)mmu:19264
General Description of CD45.
The 30-F11 antibody reacts with mouse CD45, which is one of the most abundant hematopoietic markers and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.The 30-F11 antibody is widely used as a leukocyte marker for B cells, T cell subsets and NK cell subsets.

Mouse Anti-CD45 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD45 Antibody Clone: 30-F11
Clone 30-F11 Host and Isotype: Rat IgG2b kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD45 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD45 / PTPRC antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD45 / PTPRC could be seen in. We were unable to detect CD45 / PTPRC in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD45 expression as measured by anti-CD45 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - +++ - - - - - - - - - -
PTPRC Variability + + + + + + + + + + + + + + + + + + + +

Selected References

Panchal RG, Ulrich RL, Bradfute SB, Lane D, Ruthel G, Kenny TA, Iversen PL, Anderson AO, Gussio R, Raschke WC, and Bavari S. 2009. 284: 12874-12885. (Immunoprecipitation)Cherpes TL, Busch JL, Sheridan BS, Harvey SAK, and Hendricks RL. 2008. J. Immunol. 181: 969-975. (Complement-mediated cell depletion)Cheng G, Zhang H, Yang X, Tzima E, Ewalt KL, Schimmel P, and Faber JE. 2008. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295: R1138-R1146. (Immunohistochemistry - paraffin embedded tissue)Nguyen JT, Evans DP, Galvan M, Pace KE, Leitenberg D, Bui TN, and Baum LG. 2001. J. Immunol. 167: 5697-5707. (Immunofluorescence microscopy, Immunoprecipitation)Czyzyk J, Leitenberg D, Taylor T, and Bottomly K. 2000. Mol. Cell. Biol. 20(23): 5740-5747. (Western Blot)Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273: 30680-30687. (Western Blot)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD45 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-CD45 Antibody [30-F11]”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif

enQuire Bio, LLC   1825 Lawrence St, Ste 115   Denver, CO 80202
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-855-344-1400
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email
Scroll to top

We consume cookies ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it. Dismiss

https://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB40-APC-100ug-anti-CD45-antibody-10-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB40-B-100ug-anti-CD45-antibody-10-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB40-F-100ug-anti-CD45-antibody-10-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB40-PE-100ug-anti-CD45-antibody-10-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB40-PE7-100ug-anti-CD45-antibody-10-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB40-QF710-100ug-anti-CD45-antibody-10-300x286.pnghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-QAB40-V450-100ug-anti-CD45-antibody-10-300x279.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB40-APC-100ug-anti-CD45-antibody-9-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB40-F-100ug-anti-CD45-antibody-9-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB40-PE-100ug-anti-CD45-antibody-9-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB40-PE7-100ug-anti-CD45-antibody-9-300x295.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB40-QF710-100ug-anti-CD45-antibody-9-300x286.pnghttps://enquirebio.com/wp-content/uploads/2017/11/enQuire-Bio-QAB40-V450-100ug-anti-CD45-antibody-9-300x279.png