Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RA Monoclonal Antibody (PTPRC/1148).
Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RA Monoclonal Antibody (PTPRC/1148).

Anti-CD45RA Antibody Clone PTPRC/1148

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 5788-MSM18
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
5788-MSM18-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 5788-MSM18-P1
CD45RA General Information
Alternate Names
Protein tyrosine phosphatase, receptor type, C, PTPRC
Molecular Weight
205-220kDa
Chromosomal Location
1q31.3
Curated Database and Bioinformatic Data
Gene SymbolPTPRC
Entrez Gene ID5788
Ensemble Gene IDENSG00000081237, ENSG00000262418
RefSeq Protein Accession(s)XP_006711537, XP_006711536, XP_006711535, NP_563578, NP_001254727, NP_002829
RefSeq mRNA Accession(s)XM_006711473, XM_006711474, NM_080921, NM_080922, NM_001267798, NM_002838, XM_006711472, NR_052021
RefSeq Genomic Accession(s)NG_007730, NC_000001, NW_003315907, NC_018912
UniProt ID(s)X6R433, A0A0A0MT22, P08575, M9MML4
UniGene ID(s)X6R433, A0A0A0MT22, P08575, M9MML4
HGNC ID(s)9666
Cosmic ID(s)PTPRC
KEGG Gene ID(s)hsa:5788
PharmGKB ID(s)PA34011
General Description of CD45RA.
Recognizes a protein of 205kDa-220kDa, identified as CD45RA. It is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B, C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B, O. This antibody reacts with ABC, BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells,, leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA, CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

Human Anti-CD45RA Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD45RA Antibody Clone: PTPRC/1148
Clone PTPRC/1148 Host and Isotype: Mouse IgG1 kappa
Anti-Human CD45RA Positive Control Sample: Daudi, Raji, IM9, human lymphocytes. Human lymph nodes, tonsils.
Cellular Localization of Antibody Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human CD45RA protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD45RA Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD45RA antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD45RA could be seen in. We were unable to detect CD45RA in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD45RA expression as measured by anti-CD45RA antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - +++ - - - - - - - - - -
PTPRC Variability + + + + + + + + + + + + + + + + + + + +

Limitations and Warranty

enQuire Bio's CD45RA Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD45RA Antibody Clone PTPRC/1148

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