Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RB Monoclonal Antibody (DF-B1).
Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RB Monoclonal Antibody (DF-B1).

Anti-CD45RB Antibody Clone DF-B1

$ 298.00
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SKU: 5788-MSM13
Species: Human and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG3 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
5788-MSM13-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 5788-MSM13-P1
CD45RB General Information
Alternate Names
Protein tyrosine phosphatase, receptor type, C, PTPRC
Molecular Weight
180-220kDa
Chromosomal Location
1q31.3
Curated Database and Bioinformatic Data
Gene SymbolPTPRC
Entrez Gene ID5788
Ensemble Gene IDENSG00000081237, ENSG00000262418
RefSeq Protein Accession(s)XP_006711537, XP_006711536, XP_006711535, NP_563578, NP_001254727, NP_002829
RefSeq mRNA Accession(s)XM_006711473, XM_006711474, NM_080921, NM_080922, NM_001267798, NM_002838, XM_006711472, NR_052021
RefSeq Genomic Accession(s)NG_007730, NC_000001, NW_003315907, NC_018912
UniProt ID(s)X6R433, A0A0A0MT22, P08575, M9MML4
UniGene ID(s)X6R433, A0A0A0MT22, P08575, M9MML4
HGNC ID(s)9666
Cosmic ID(s)PTPRC
KEGG Gene ID(s)hsa:5788
PharmGKB ID(s)PA34011
General Description of CD45RB.
CD45R, also designated CD45, PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5,, 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6), CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes, the majority of lymphomas, leukemias of B-cell origin. This antigen is also expressed on the surface of cells of myeloid lineage.

Human and Rat (-) Anti-CD45RB Antibody Product Attributes

Species: Human and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD45RB Antibody Clone: DF-B1
Clone DF-B1 Host and Isotype: Mouse IgG3 kappa
Anti-Human and Rat (-) CD45RB Positive Control Sample: Ramos, U-698, or GA-10 cells. Tonsil
Cellular Localization of Antibody Cell surface, cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Extract from Alexander’s hepatoma cell line L428 (Workshop V)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD45RB Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD45RB antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD45RB could be seen in. We were unable to detect CD45RB in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD45RB expression as measured by anti-CD45RB antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - +++ - - - - - - - - - -
PTPRC Variability + + + + + + + + + + + + + + + + + + + +

Limitations and Warranty

enQuire Bio's CD45RB Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD45RB Antibody Clone DF-B1

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