Flow Cytometry of hPBMC using CD45RO Monoclonal Antibody (UCHL-1).
Flow Cytometry of hPBMC using CD45RO Monoclonal Antibody (UCHL-1).

Anti-CD45RO Antibody Clone UCHL-1

$ 298.00
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SKU: 5788-MSM1
Species: Human, Chimpanzee, and Common Marmoset
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
5788-MSM1-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 5788-MSM1-P1
CD45RO General Information
Alternate Names
Protein tyrosine phosphatase, receptor type, C, PTPRC
Molecular Weight
180-185kDa
Chromosomal Location
1q31.3
Curated Database and Bioinformatic Data
Gene SymbolPTPRC
Entrez Gene ID5788
Ensemble Gene IDENSG00000081237, ENSG00000262418
RefSeq Protein Accession(s)XP_006711537, XP_006711536, XP_006711535, NP_563578, NP_001254727, NP_002829
RefSeq mRNA Accession(s)XM_006711473, XM_006711474, NM_080921, NM_080922, NM_001267798, NM_002838, XM_006711472, NR_052021
RefSeq Genomic Accession(s)NG_007730, NC_000001, NW_003315907, NC_018912
UniProt ID(s)X6R433, A0A0A0MT22, P08575, M9MML4
UniGene ID(s)X6R433, A0A0A0MT22, P08575, M9MML4
HGNC ID(s)9666
Cosmic ID(s)PTPRC
KEGG Gene ID(s)hsa:5788
PharmGKB ID(s)PA34011
General Description of CD45RO.
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes,, a sub-population of resting T-cells within both CD4, CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes, monocytes. Reportedly, it is useful to identify T-cell lymphomas, leukemias. It rarely stains NK cells or B-cell lymphomas.

Human, Chimpanzee, and Common Marmoset Anti-CD45RO Antibody Product Attributes

Species: Human, Chimpanzee, and Common Marmoset
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD45RO Antibody Clone: UCHL-1
Clone UCHL-1 Host and Isotype: Mouse IgG2a kappa
Anti-Human, Chimpanzee, and Common Marmoset CD45RO Positive Control Sample: CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Cultured human T-cells from an IL-2-dependent T-cell line (CA1)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD45RO Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD45RO antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, germinal center cells in the lymph node, non-germinal center cells in the lymph node, cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of CD45RO could be seen in. We were unable to detect CD45RO in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD45RO expression as measured by anti-CD45RO antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - +++ - - - - - - - - - -
PTPRC Variability + + + + + + + + + + + + + + + + + + + +

Limitations and Warranty

enQuire Bio's CD45RO Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD45RO Antibody Clone UCHL-1

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