Mouse Anti-CD5 Antibody Product Attributes
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD5 Antibody Clone: 53-7.3
Clone 53-7.3 Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD5 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in CD5 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD5 could be seen in. We were unable to detect CD5 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Azzam HS, Grinberg A, Lui K, Shen H, Shores EW, Love PE. 1998. J Exp Med. 188(12):2301-2311.Bikah G, Carey J, Ciallella JR, Tarakhovsky A, Bondada S. 1996. Science. 274(5294):1906-1909.Bikah G, Lynd FM, Aruffo AA, Ledbetter JA, Bondada S. 1998. Int Immunol. 10(8):1185-1196.Hayakawa K, Hardy RR, Parks DR, Herzenberg LA. 1983. J Exp Med. 157(1):202-218.Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. 1980. J Exp Med. 152(2):280-295.Tarakhovsky A, Kanner SB, Hombach J, et al. 1995. Science. 269(5223):535-537.
Limitations and Warranty
enQuire Bio's Mouse Anti-CD5 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.