Human Anti-CD5 Antibody Product Attributes
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD5 Antibody Clone: L17F12
Clone L17F12 Host and Isotype: Mouse IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD5 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in CD5 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the white pulp in spleen and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD5 could be seen in. We were unable to detect CD5 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Knapp W, Dorken B, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.Gadol N and Ault KA. 1986. Immunol Rev. 93: 23-34.Antin JH, Emerson SG, Martin P, Gadol N and Ault KA. 1986. J Immunol. 136: 505-510.Raab M, Yamamoto M and Rudd CE. 1994. Mol Cell Biol. 14(5): 2862-2870.Lydyard PM, Lamour A, MacKenzie LE, Jamin C, Magged RA and Youinou P. 1993. Immunol Lett. 38(2): 159-166.McKenna RW, Washington LT, Aquino DB, Picker LJ and Kroft SH. 2001. Blood. 98(8): 2498-2507. (Flow Cytometry)Butmarc JR, Kourea HP, Levi E and Kadin ME. 1998. Am J Clin Path. 109(6): 682-688. (Immunohistochemistry - paraffin-embedded tissue)
Limitations and Warranty
enQuire Bio's Human Anti-CD5 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.