Anti-CD50 / ICAM-3 Antibody Clone SPM505

$ 298.00
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SKU: 3385-MSM1X
Species: Human
Tested Applications: Bioactive, Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
3385-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 3385-MSM1X-P1
CD50 / ICAM-3 General Information
Alternate Names
Intercellular adhesion molecule 3, ICAM3, CD50, Cluster of Differentiation 50, ICAM-3
Molecular Weight
110-160kDa
Chromosomal Location
19p13.2
Curated Database and Bioinformatic Data
Gene SymbolICAM3
Entrez Gene ID3385
Ensemble Gene IDENSG00000076662
RefSeq Protein Accession(s)NP_001307534, NP_001307537, NP_001307535, NP_002153
RefSeq mRNA Accession(s)NM_001320606, NM_001320608, NM_002162, NM_001320605
RefSeq Genomic Accession(s)NC_000019, NC_018930
UniProt ID(s)A0A024R7C1, K7ERN2, P32942
UniGene ID(s)A0A024R7C1, K7ERN2, P32942
HGNC ID(s)5346
Cosmic ID(s)ICAM3
KEGG Gene ID(s)hsa:3385
PharmGKB ID(s)PA29594
General Description of CD50 / ICAM-3.
Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18), may have signaling role to increase adhesion. It is expressed on thymocytes, T lymphocytes, is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.

Human Anti-CD50 / ICAM-3 Antibody Product Attributes

Species: Human
Tested Applications: Bioactive, Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3), Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD50 / ICAM-3 Antibody Clone: SPM505
Clone SPM505 Host and Isotype: Mouse IgG2b kappa
Anti-Human CD50 / ICAM-3 Positive Control Sample: HL-60 or THP-1 cells. Lymph node, tonsil
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Stimulated human leukocytes
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD50 / ICAM-3 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-ICAM3 antibody staining is expected to be primarily localized to the mitochondria, nuclear membrane and nucleus.

Observed Antibody Staining Data By Tissue Type:

Variations in CD50 / ICAM-3 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen, cells in the white pulp in spleen, epidermal cells in the skin, germinal center cells in the tonsil, hematopoietic cells in the bone marrow, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen, cells in the white pulp in spleen, epidermal cells in the skin, germinal center cells in the tonsil, hematopoietic cells in the bone marrow, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD50 / ICAM-3 could be seen in. We were unable to detect CD50 / ICAM-3 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD50 / ICAM-3 expression as measured by anti-CD50 / ICAM-3 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - +++ - - - - - - - - - -
ICAM3 Variability + + + + + + + + + + + + + + + + + + + ++

Limitations and Warranty

enQuire Bio's CD50 / ICAM-3 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD50 / ICAM-3 Antibody Clone SPM505

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