FACS analysis of CD56 on human Monocytes using CD56 Monoclonal Antibody (SPM128)
FACS analysis of CD56 on human Monocytes using CD56 Monoclonal Antibody (SPM128)

Anti-CD56 / NCAM1 Antibody Clone SPM128

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 4684-MSM1X
Species: Human, Rat, and Zebrafish
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
4684-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 4684-MSM1X-P1
CD56 / NCAM1 General Information
Alternate Names
Neural cell adhesion molecule, NCAM, CD56
Molecular Weight
180, 145, 125kDa
Chromosomal Location
11q23.1
Curated Database and Bioinformatic Data
Gene SymbolNCAM1
Entrez Gene ID4684
Ensemble Gene IDENSG00000149294
RefSeq Protein Accession(s)NP_001229536, NP_851996, NP_001070150, NP_001229537, NP_000606
RefSeq mRNA Accession(s)NM_001076682, NM_001242607, NM_001242608, NM_181351 NM_000615
RefSeq Genomic Accession(s)NC_000011, NG_032036, NC_018922
UniProt ID(s)P13591, A0A087WWD4
UniGene ID(s)P13591, A0A087WWD4
HGNC ID(s)7656
Cosmic ID(s)NCAM1
KEGG Gene ID(s)hsa:4684
PharmGKB ID(s)PA31459
General Description of CD56 / NCAM1.
This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues, neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas,, small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal, nasal NK/T-cell lymphomas.

Human, Rat, and Zebrafish Anti-CD56 / NCAM1 Antibody Product Attributes

Species: Human, Rat, and Zebrafish
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD56 / NCAM1 Antibody Clone: SPM128
Clone SPM128 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Rat, and Zebrafish CD56 / NCAM1 Positive Control Sample: Cerebellum, Pancreas, Neuroblastoma
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Membrane preparation of a small cell lung carcinoma
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD56 / NCAM1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-NCAM1 antibody staining is expected to be primarily localized to the cytosol and plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in CD56 / NCAM1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in neuropil in cerebral cortex and peripheral nerve/ganglion in colon. More moderate antibody staining intensity was present in neuropil in cerebral cortex and peripheral nerve/ganglion in colon. Low, but measureable presence of CD56 / NCAM1 could be seen inglial cells in the hippocampus, exocrine glandular cells in the pancreas and smooth muscle cells in the smooth muscle. We were unable to detect CD56 / NCAM1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD56 / NCAM1 expression as measured by anti-CD56 / NCAM1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - ++ - - - - - - - - - - - - - -
NCAM1 Variability + ++ + + + ++ + + + + + ++ + + + + + + ++ +

Limitations and Warranty

enQuire Bio's CD56 / NCAM1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD56 / NCAM1 Antibody Clone SPM128

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