Formalin-fixed, paraffin-embedded human Tonsil stained with CD57 Monoclonal Antibody (NK/84).

Anti-CD57 / B3GAT1 Antibody Clone NK/804

$ 429.00
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SKU: 27087-MSM4
Species: Human and Rat (-)
Tested Applications: Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgM kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
27087-MSM4-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 27087-MSM4-P1
CD57 / B3GAT1 General Information
Alternate Names
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, B3GAT1,
Molecular Weight
Chromosomal Location
Curated Database and Bioinformatic Data
Gene SymbolB3GAT1
Entrez Gene ID27087
Ensemble Gene IDENSG00000109956
RefSeq Protein Accession(s)XP_016873039, XP_005271563, NP_473366, XP_016873041, XP_011541053, XP_016873040, XP_011541055, NP_061114
RefSeq mRNA Accession(s)NM_018644, XM_005271506, XM_017017550, XM_011542753, XM_011542751, XM_017017551, NM_054025, XM_017017552
RefSeq Genomic Accession(s)NC_018922, NC_000011
UniProt ID(s)Q9P2W7
UniGene ID(s)Q9P2W7
HGNC ID(s)921
Cosmic ID(s)B3GAT1
KEGG Gene ID(s)hsa:27087
PharmGKB ID(s)PA25215
General Description of CD57 / B3GAT1.
Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells, their derived tumors, including carcinoid tumor, medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5,, CEA.

Human and Rat (-) Anti-CD57 / B3GAT1 Antibody Product Attributes

Species: Human and Rat (-)
Tested Applications: Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD57 / B3GAT1 Antibody Clone: NK/804
Clone NK/804 Host and Isotype: Mouse IgM kappa
Anti-Human and Rat (-) CD57 / B3GAT1 Positive Control Sample: Lymph node or Tonsil
Cellular Localization of Antibody Cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human B3GAT1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

CD57 / B3GAT1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-B3GAT1 antibody staining is expected to be primarily localized to the vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in CD57 / B3GAT1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glial cells in the caudate nucleus and cerebral cortex, neuronal cells in the cerebral cortex, neuropil in cerebral cortex and peripheral nerve in mesenchymal tissue. More moderate antibody staining intensity was present in glial cells in the caudate nucleus and cerebral cortex, neuronal cells in the cerebral cortex, neuropil in cerebral cortex and peripheral nerve in mesenchymal tissue. Low, but measureable presence of CD57 / B3GAT1 could be seen in cells in the red pulp in spleen, cells in the tubules in kidney, cells in the white pulp in spleen, germinal center cells in the lymph node and tonsil, glandular cells in the adrenal gland, appendix, colon, duodenum, prostate, rectum, small intestine and stomach, non-germinal center cells in the lymph node and tonsil and Purkinje cells in the cerebellum. We were unable to detect CD57 / B3GAT1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD57 / B3GAT1 expression as measured by anti-CD57 / B3GAT1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - + - - - ++ - - - - - - - ++ - - - - - -
B3GAT1 Variability + +++ + + + ++ + + + + + + + + + + + + + +

Limitations and Warranty

enQuire Bio's CD57 / B3GAT1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD57 / B3GAT1 Antibody Clone NK/804