Human Anti-CD59 / Complement Regulatory Protein / Protectin Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-CD59 / Complement Regulatory Protein / Protectin Antibody Clone: MACIF/629
Clone MACIF/629 Host and Isotype: Mouse IgG1 kappa
Anti-Human CD59 / Complement Regulatory Protein / Protectin Positive Control Sample: Daudi, CEM, K562, HPB.ALL, Jurkat, Raji,, human lymphocytes. Tonsil, Cervix or Bladder.
Cellular Localization of Antibody Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human CD59 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
CD59 / Complement Regulatory Protein / Protectin Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-CD59 antibody staining is expected to be primarily localized to the golgi apparatus and vesicles.Observed Antibody Staining Data By Tissue Type:
Variations in CD59 / Complement Regulatory Protein / Protectin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in endothelial cells in the colon and peripheral nerve/ganglion in colon. More moderate antibody staining intensity was present in endothelial cells in the colon and peripheral nerve/ganglion in colon. Low, but measureable presence of CD59 / Complement Regulatory Protein / Protectin could be seen inbile duct cells in the liver, cells in the granular layer in cerebellum, cells in the seminiferous ducts in testis, cells in the tubules in kidney, exocrine glandular cells in the pancreas, fibroblasts in mesenchymal tissue, germinal center cells in the tonsil, glandular cells in the endometrium and stomach, glial cells in the caudate nucleus, keratinocytes in skin, macrophages in lung, melanocytes in skin, myocytes in skeletal muscle, neuropil in cerebral cortex, non-germinal center cells in the lymph node, squamous epithelial cells in the cervix and uterine and oral mucosa. We were unable to detect CD59 / Complement Regulatory Protein / Protectin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of CD59 / Complement Regulatory Protein / Protectin expression as measured by anti-CD59 / Complement Regulatory Protein / Protectin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | + | - | - | - | + | - | ++ | + | - | - | ++ | - | - | - | - | - | + | - | ++ | - |
| CD59 Variability | ++ | ++ | ++ | ++ | +++ | + | ++ | +++ | ++ | + | ++ | ++ | ++ | + | + | ++ | ++ | + | ++ | ++ |
Limitations and Warranty
enQuire Bio's CD59 / Complement Regulatory Protein / Protectin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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