IF staining of HeLa cells using AF488 labeled CD63 MAb (MX-49.129.5) (Green). F-actin filaments are labeled with Dylight 554 phalloidin (red). DAPI was used to stain the cell nucleus (blue).

Anti-CD63 Antibody Clone MX-49.129.5

Name: Anti-CD63 Antibody Clone MX-49.129.5
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 429.00

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SKU: 967-MSM2
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
967-MSM2-P1	Size: 100 ug, Tag: Unconjugated

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In Stock.

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Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 967-MSM2-P1

CD63 General Information
Alternate Names
CD63 antigen, CD63, Cluster of Differentiation 63
Molecular Weight
26kDa (core protein); 30-60kDa (glycosylated)
Chromosomal Location
12q13.2
Curated Database and Bioinformatic Data
Gene Symbol	CD63
Entrez Gene ID	967
Ensemble Gene ID	ENSG00000135404
RefSeq Protein Accession(s)	NP_001244320, NP_001254627, NP_001771, NP_001244321, NP_001244329, NP_001244319, NP_001244330, NP_001244318
RefSeq mRNA Accession(s)	NM_001780, NM_001040034, NM_001257401, NM_001267698, NM_001257392, NM_001257391, NM_001257400, NM_001257389, NM_001257390
RefSeq Genomic Accession(s)	NG_008347, NC_018923, NC_000012
UniProt ID(s)	P08962, A0A024RB05
UniGene ID(s)	P08962, A0A024RB05
HGNC ID(s)	1692
Cosmic ID(s)	CD63
KEGG Gene ID(s)	hsa:967
PharmGKB ID(s)	PA26231
General Description of CD63.
This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface, granular membranes of hematopoietic cells, are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes, macrophages,, is weakly expressed on granulocytes, T cell, B cells. It is located on the basophilic granule membranes, on the plasma membranes of lymphocytes, granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37, CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic, intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome, is strongly expressed during the early stages of melanoma progression.

Human and Mouse Anti-CD63 Antibody Product Attributes

Species: Human and Mouse

Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).

Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-CD63 Antibody Clone: MX-49.129.5

Clone MX-49.129.5 Host and Isotype: Mouse IgG1 kappa

Anti-Human and Mouse CD63 Positive Control Sample: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.

Cellular Localization of Antibody Cell Surface & Cytoplasmic

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Full length CD63 of human origin

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

CD63 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CD63 antibody staining is expected to be primarily localized to the vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in CD63 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD63 could be seen in cells in the seminiferous ducts in testis, cells in the tubules in kidney, decidual cells in the placenta, endothelial cells in the colon, exocrine glandular cells in the pancreas, glandular cells in the adrenal gland, appendix, colon, duodenum, endometrium, parathyroid gland and small intestine, hepatocytes in liver, islets of Langerhans in pancreas, melanocytes in skin, myocytes in heart muscle, non-germinal center cells in the lymph node and tonsil, peripheral nerve/ganglion in colon, respiratory epithelial cells in the bronchus and squamous epithelial cells in the tonsil. We were unable to detect CD63 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD63 expression as measured by anti-CD63 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	-	-	-	-	+	-	-	-	+	-	+++	+	+	+	+	-	-	-	+	-
CD63 Variability	++	++	++	++	++	+	+	++	+++	++	++	++	++	++	++	+	++	++	+	++

Limitations and Warranty

enQuire Bio's CD63 Anti-Human, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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