C57Bl/6 splenocytes were stimulated overnight with ConA and then  stained with 0.25 ug PE-Cy7 Mouse Anti-CD69 .

Anti-CD69 Antibody [H1.2F3]

$ 68.00$ 168.00
Please Select Product Options Below To View The Catalog Number.

Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;PE-Cy7
Isotype: Armenian Hamster IgG
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
QAB90-F-25ug Size: 25 ug, Tag: FITC $ 68.00
QAB90-F-100ug Size: 100 ug, Tag: FITC $ 118.00
QAB90-PE7-100ug Size: 100 ug, Tag: PE-Cy7 $ 168.00
QAB90-PE7-25ug Size: 25 ug, Tag: PE-Cy7 $ 68.00
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In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
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Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

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Bioactivity (test results eg. IU/ml):

SKU: QAB90-F-25ug
CD69 / CLEC2C / AIM General Information
Alternate Names
CD69, CLEC2C, AIM, BL-AC/P26, CLEC2C, GP32/28, AIM, EA1, MLR-3
Curated Database and Bioinformatic Data
Gene SymbolCd69
Entrez Gene ID12515
Ensemble Gene IDENSMUSG00000030156
RefSeq Protein Accession(s)XP_006505526, NP_001028294
RefSeq mRNA Accession(s)XM_006505463, NM_001033122
RefSeq Genomic Accession(s)NC_000072
UniProt ID(s)P37217, Q3U6A8
UniGene ID(s)P37217, Q3U6A8
Cosmic ID(s)Cd69
KEGG Gene ID(s)mmu:12515
General Description of CD69 / CLEC2C / AIM.
The H1.2F3 antibody reacts with mouse CD69, a type II transmembrane glycoprotein also known as the Very Early Activation Antigen, EA-1, Leu23, Activation Inducer Molecule (AIM) and CLEC2C. CD69 is expressed as a 60 kDa disulfide-linked homodimer on activated T and B cells, NK cells, neutrophils and monocytes. Induction occurs rapidly upon activation. It is also constitutively expressed on platelets and a subset of thymocytes. CD69 acts as a co-stimulatory molecule involved in activation and proliferation of T cells, and may be a marker for thymocytes undergoing TCR-mediated positive selection.Co-stimulation with the H1.2F3 clone has been reported to enhance T cell and macrophage activation. Please choose the appropriate format for each application.

Mouse Anti-CD69 / CLEC2C / AIM Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD69 / CLEC2C / AIM Antibody Clone: H1.2F3
Clone H1.2F3 Host and Isotype: Armenian Hamster IgG
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD69 / CLEC2C / AIM Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD69 / CLEC2C / AIM antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD69 / CLEC2C / AIM could be seen in cells in the red pulp in spleen, cells in the white pulp in spleen, germinal center cells in the lymph node, hematopoietic cells in the bone marrow and non-germinal center cells in the lymph node. We were unable to detect CD69 / CLEC2C / AIM in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Yamashita I, Nagata T, Tada T and Nakayam T. 1993. Int Immunol. 5(9): 1139-1150.Ziegler SF, Ramsdell F and Alderson MR. 1994. Stem Cells. 12(5): 456-465. Marzio R, Jirillo E, Ransijn A, Mauel J and Corradin SB. 1997. J Leukoc Biol. 62(3): 349-355.Mackay LK, Braun A, Macleaod BL, Collins N, Tebartz C, Bedoui S, Carbone FR and Gebhardt T. 2015. J Immunol. 194(5): 2059-2063. (Flow Cytometry)Radulovic K, Manta C, Rossini V, Holzmann K, Kestler HA, Wegenka UM, Nakayam T and Niess JH. 2012. J Immunol. 188(4): 2001-2013. (Flow Cytometry, in vitro activation)Bremser A, Brack M and Izcue A. 2015. PLoS One. 10(9):e0137393. (Flow Cytometry)Zhou X, Li F, Kong L, Tomita H, Li C and Cao W. 2005. J Biol Chem. 280(35): 31240-31248. (Immunofluorescence Microscopy)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD69 Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-CD69 Antibody [H1.2F3]