Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Ab (SPM523).

Anti-CD74 Antibody Clone SPM523

Name: Anti-CD74 Antibody Clone SPM523
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 429.00

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SKU: 972-MSM1X
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
972-MSM1X-P1	Size: 100 ug, Tag: Unconjugated

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Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 972-MSM1X-P1

CD74 General Information
Alternate Names
CLIP, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, CD74, Cluster of Differentiation 74
Molecular Weight
33-41kDa
Chromosomal Location
5q33.1
Curated Database and Bioinformatic Data
Gene Symbol	CD74
Entrez Gene ID	972
Ensemble Gene ID	ENSG00000019582
RefSeq Protein Accession(s)	NP_001020329, NP_001020330, NP_004346, XP_016865579, XP_016865578
RefSeq mRNA Accession(s)	NM_004355 NM_001025158, NM_001025159, XM_017010089, XM_017010090
RefSeq Genomic Accession(s)	NG_029730, NC_018916, NC_000005
UniProt ID(s)	P04233
UniGene ID(s)	P04233
HGNC ID(s)	1697
Cosmic ID(s)	CD74
KEGG Gene ID(s)	hsa:972
PharmGKB ID(s)	PA26236
General Description of CD74.
CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers, prevents their premature association with enCanineenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages,, monocytes,, many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes, B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).

Human and Mouse Anti-CD74 Antibody Product Attributes

Species: Human and Mouse

Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).

Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-CD74 Antibody Clone: SPM523

Clone SPM523 Host and Isotype: Mouse IgG1 kappa

Anti-Human and Mouse CD74 Positive Control Sample: Daudi Cells. Tonsil or lymph node.

Cellular Localization of Antibody Cell surface, paranuclear globular

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: SU-DHL-4 lymphoma cells

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

CD74 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CD74 antibody staining is expected to be primarily localized to the golgi apparatus. There is variability in either the signal strength or the localization of signal in golgi apparatus from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in CD74 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD74 could be seen in cells in the molecular layer in cerebellum, cells in the tubules in kidney, endothelial cells in the cerebral cortex, follicle cells in the ovary, glandular cells in the appendix, cervix, uterine, endometrium, small intestine, stomach and thyroid gland, glial cells in the caudate nucleus, cerebral cortex and hippocampus, hematopoietic cells in the bone marrow, Leydig cells in the testis, neuronal cells in the caudate nucleus, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the esophagus and trophoblastic cells in the placenta. We were unable to detect CD74 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of CD74 expression as measured by anti-CD74 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	-	-	++	+	++	+	++	+	+	++	-	+	-	+	+	-	-	-	++	-
CD74 Variability	++	+	++	++	++	++	++	++	++	+	++	+++	++	++	++	++	++	+	++	++

Limitations and Warranty

enQuire Bio's CD74 Anti-Human, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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