Human and Mouse Anti-CD74 Antibody Product Attributes
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Anti-CD74 Antibody Clone: SPM523
Clone SPM523 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Mouse CD74 Positive Control Sample: Daudi Cells. Tonsil or lymph node.
Cellular Localization of Antibody
Cell surface, paranuclear globular
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: SU-DHL-4 lymphoma cells
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
CD74 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:Anti-CD74 antibody staining is expected to be primarily localized to the golgi apparatus. There is variability in either the signal strength or the localization of signal in golgi apparatus from cell to cell.
Observed Antibody Staining Data By Tissue Type:Variations in CD74 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of CD74 could be seen in cells in the molecular layer in cerebellum, cells in the tubules in kidney, endothelial cells in the cerebral cortex, follicle cells in the ovary, glandular cells in the appendix, cervix, uterine, endometrium, small intestine, stomach and thyroid gland, glial cells in the caudate nucleus, cerebral cortex and hippocampus, hematopoietic cells in the bone marrow, Leydig cells in the testis, neuronal cells in the caudate nucleus, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the esophagus and trophoblastic cells in the placenta. We were unable to detect CD74 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of CD74 expression as measured by anti-CD74 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Limitations and Warranty
enQuire Bio's CD74 Anti-Human, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
There are no reviews yet.