C57Bl/6 splenocytes were stained with 0.125 ug Mouse Anti-C8a APC (QAB61) (solid line) or 0.125 ug Rat IgG2b APC isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Mouse Anti-CD8a Antibody [2.43]

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Species: Mouse
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;APC ;PerCP-Cy5.5 ;PE ;PE-Cy7 ;V450
Isotype: Rat IgG2b
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB61-F-25ug
CD8 General Information
Alternate Names
CD8A, MAL, CD8, p32, Leu2, MAL
Curated Database and Bioinformatic Data
Gene SymbolCd8a
Entrez Gene ID12525
Ensemble Gene IDENSMUSG00000053977
RefSeq Protein Accession(s)NP_001074579, NP_033987
RefSeq mRNA Accession(s)NM_009857, NM_001081110
RefSeq Genomic Accession(s)NC_000072
UniProt ID(s)Q8CAX3, P01731
UniGene ID(s)Q8CAX3, P01731
Cosmic ID(s)Cd8a
KEGG Gene ID(s)mmu:12525
General Description of CD8.
The 2.43 antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation induced by binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck and facilitate intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death in tumor cells, as well as in cells infected by virus, bacteria or parasites.The 2.43 antibody is widely used as a phenotypic marker for mouse CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.

Mouse Anti-CD8 Antibody Product Attributes

Species: Mouse
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD8 Antibody Clone: 2.43
Clone 2.43 Host and Isotype: Rat IgG2b
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.5 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD8 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD8 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen, cells in the white pulp in spleen, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen, cells in the white pulp in spleen, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD8 could be seen inmacrophages in lung. We were unable to detect CD8 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Lin J-S, Szaba FM, Kummer LW, Chromy BA, and Smiley ST. 2011. J. Immunol. 187: 897-904. (in vivo depletion)Wozniak KL, Young ML, and Wormley FL. 2011. Clin. Vaccine Immunol. 18(5):717-723. (in vivo depletion)Hufford MM, Kim TS, Sun J, and Braciale TJ. 2011. J. Exp. Med. 208: 167-180 (in vivo depletion)Ou R, zhang M, Huang L, Flavell RA, Koni PA, and Moskophidis D. 2008. J. Virol. 82:2952-2965. (Immunohistochemistry - OCT embedded frozen tissue)Bosselut R, Zhang W, Ashe JM, Kopacz JL, Samelson LE, and Singer A. 1999. J. Exp. Med. 190: 1517-1526. (in vitro activation)Davies A, Kalb, S, Liang B, Aldrich CJ, Lemonnier FA, Jiang H, Cotter R, and Soloski MJ. 2003. J. Immunol. 170: 5027-5033. (Blocking)

Limitations and Warranty

enQuire Bio's Mouse Anti-CD8a Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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