Mouse Anti-CD8 Antibody Product Attributes
Tested Applications: Flow Cytometry.
Application Notes: See Product Datasheet for Recommended Dilution Range. Requires Experimental Optimization
Clonality: Monoclonal Antibody
Anti-CD8 Antibody Clone: 53-6.7
Clone 53-6.7 Host and Isotype: Rat IgG2a kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 0.2 mg/mL
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD8 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD8 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen, cells in the white pulp in spleen, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen, cells in the white pulp in spleen, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD8 could be seen inmacrophages in lung. We were unable to detect CD8 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Willinger T and Flavell RA. 2012. Proc. Natl. Acad. Sci. 109:8670-8675. (Flow cytometry)Thaventhiran JED, Hoffmann A, Magiera L, de la Roche M, Lingel H, Brunner-Weinzierl M, and Fearon DT. 2012. Proc. Natl. Acad. Sci. 10.1073. (Immunohistochemistry - OCT embedded frozen tissue)Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, and Ishida S. 2008. Invest. Ophthalmol. Vis. Sci. 49(5):2172-2127. (in vivo cell depletion)Fan K, Zhou M, Pathak MK, Lindner DJ, Altuntas CZ, Touhy VK, Borden EC, and Yi T. 2005. J. Immunol. 175:7003-7008. (Immunohistochemistry - frozen tissue)Nutt SL, Metcalf D, DÂAmico A, Polli M, and Wu L. 2005. J. Exp. Med. 201:221-231. (Immunomagnetic bead depletion)Fan G-C, and Singh, RR. 2002. J. Exp. Med. 196: 731-741. (in vitro cell depletion)Bosselut R, Zhang W, Ashe JM, Kopacz JL, Samelson LE, and Singer A. 1999. J. Exp. Med. 190: 1517-1526. (Immunoprecipitation)
Limitations and Warranty
enQuire Bio's Mouse Anti-CD8a Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.