chimpanzee, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD8 Antibody Clone: Hit8a
Clone Hit8a Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.
CD8 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in CD8 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen, cells in the white pulp in spleen, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen, cells in the white pulp in spleen, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD8 could be seen inmacrophages in lung. We were unable to detect CD8 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Coppieters KT, Dotta F, Amirian N, Campbell PD, Kay TWH, Atkinson MA, Roep BO and von Herrath MG. 2012. J. Exp. Med. 109: 51-60. (in situ tetramer staining / Immunohistochemistry - frozen tissue)Kumari A, Brendel C, Hochlaus A, Neubauer A, and Burchert A. 2012. Blood. 119: 530-539. (Flow cytometry)Kryczek I, Banerjee M, Cheng P, Vatan L, et al. 2009. Blood. 114: 1141 - 1149. (Immunofluorescence microscopy)
Limitations and Warranty
enQuire Bio's Human Anti-CD8a Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.