Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.
Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  (solid line) or 1 ug V450 Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  (solid line) or 0.125 ug APC Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.Human PBMCs were stained with 5 uL  (solid line) or 1 ug V450 Mouse IgG1 isotype control (dashed line). Flow Cytometry Data from 10,000 events.

Anti-CD8a Antibody [Hit8a]

$ 48.00$ 188.00
Please Select Product Options Below To View The Catalog Number.

SKU: QAB20
Species: Human
Tested Applications: Flow Cytometry
Available Conjugates: FITC ;Unconjugated ;APC ;V450
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: QAB20-F-25Tests
CD8 General Information
Alternate Names
CD8A, MAL, CD8, p32, Leu2, MAL
Curated Database and Bioinformatic Data
Gene SymbolCD8A
Entrez Gene ID925
Ensemble Gene IDENSG00000153563
RefSeq Protein Accession(s)NP_001759, NP_001139345, NP_741969
RefSeq mRNA Accession(s)NM_001145873, NM_001768, NM_171827, NR_027353,
RefSeq Genomic Accession(s)NC_000002, NG_011608, NC_018913
UniProt ID(s)P01732, Q8TAW8, Q6ZVS2
UniGene ID(s)P01732, Q8TAW8, Q6ZVS2
HGNC ID(s)1706
Cosmic ID(s)CD8A
KEGG Gene ID(s)hsa:925
PharmGKB ID(s)PA26244
General Description of CD8.
The Hit8a antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites. The Hit8a antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. If used together with an alternative Human Anti-CD8a clone RPA-T8, the Hit8a antibody will not block binding of RPA-T8 to CD8a.

Human Anti-CD8 Antibody Product Attributes

Species: Human
Cross Reactivity: chimpanzee, rhesus macaque
Tested Applications: Flow Cytometry.
Application Notes: 5 ul per test where one test represents staining of a cell sample in a final volume of approximately 100 uL. The number of cells within a sample must be experimentally determined, but ranges between 1x105 to 1x108 cells.
Clonality: Monoclonal Antibody
Anti-CD8 Antibody Clone: Hit8a
Clone Hit8a Host and Isotype: Mouse IgG1 kappa
Buffer and Stabilizer: 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
Antibody Concentration: 5 uL /test
Storage Conditions: 2-8C protected from light. Stable for 12 Months. Do Not Freeze.

CD8 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in CD8 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the red pulp in spleen, cells in the white pulp in spleen, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. More moderate antibody staining intensity was present in cells in the red pulp in spleen, cells in the white pulp in spleen, lymphoid tissue in appendix and non-germinal center cells in the lymph node and tonsil. Low, but measureable presence of CD8 could be seen inmacrophages in lung. We were unable to detect CD8 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Selected References

Coppieters KT, Dotta F, Amirian N, Campbell PD, Kay TWH, Atkinson MA, Roep BO and von Herrath MG. 2012. J. Exp. Med. 109: 51-60. (in situ tetramer staining / Immunohistochemistry - frozen tissue)Kumari A, Brendel C, Hochlaus A, Neubauer A, and Burchert A. 2012. Blood. 119: 530-539. (Flow cytometry)Kryczek I, Banerjee M, Cheng P, Vatan L, et al. 2009. Blood. 114: 1141 - 1149. (Immunofluorescence microscopy)

Limitations and Warranty

enQuire Bio's Human Anti-CD8a Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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